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Probing the Therapeutic Potential of Marine Phyla by SPE Extraction

Authors :
Genoveffa Nuzzo
Valerio Mazzella
Clementina Sansone
Angela Sardo
Marte Albrigtsen
Antonella Iuliano
Fabio Crocetta
Jeanette Hammer Andersen
Angelo Fontana
Christian Galasso
Giusi Barra
Carmela Gallo
Giuliana d'Ippolito
Alejandro Moreiras-Figueruelo
Adrianna Ianora
Emiliano Manzo
Moreiras-Figueruelo, Alejandro
Nuzzo, Genoveffa
Galasso, Christian
Sansone, Clementina
Crocetta, Fabio
Mazzella, Valerio
Gallo, Carmela
Barra, Giusi
Sardo, Angela
Iuliano, Antonella
Manzo, Emiliano
D'Ippolito, Giuliana
Albrigtsen, Marte
Andersen, Jeanette H
Ianora, Adrianna
Fontana, Angelo
Source :
Marine Drugs, Vol 19, Iss 640, p 640 (2021), Marine Drugs, Volume 19, Issue 11
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

The marine environment is potentially a prolific source of small molecules with significant biological activities. In recent years, the development of new chromatographic phases and the progress in cell and molecular techniques have facilitated the search for marine natural products (MNPs) as novel pharmacophores and enhanced the success rate in the selection of new potential drug candidates. However, most of this exploration has so far been driven by anticancer research and has been limited to a reduced number of taxonomic groups. In this article, we report a test study on the screening potential of an in-house library of natural small molecules composed of 285 samples derived from 57 marine organisms that were chosen from among the major eukaryotic phyla so far represented in studies on bioactive MNPs. Both the extracts and SPE fractions of these organisms were simultaneously submitted to three different bioassays—two phenotypic and one enzymatic—for cytotoxic, antidiabetic, and antibacterial activity. On the whole, the screening of the MNP library selected 11 potential hits, but the distribution of the biological results showed that SPE fractionation increased the positive score regardless of the taxonomic group. In many cases, activity could be detected only in the enriched fractions after the elimination of the bulky effect due to salts. On a statistical basis, sponges and molluscs were confirmed to be the most significant source of cytotoxic and antimicrobial products, but other phyla were found to be effective with the other therapeutic targets.

Details

Language :
English
ISSN :
16603397
Volume :
19
Issue :
640
Database :
OpenAIRE
Journal :
Marine Drugs
Accession number :
edsair.doi.dedup.....a92756f4a65a40bfc725a2304d268e35