Back to Search
Start Over
Effects of photobiomodulation on the redox state of healthy and cancer cells
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Biomed Opt Express
- Publication Year :
- 2021
- Publisher :
- Optica Publishing Group, 2021.
-
Abstract
- Photobiomodulation therapy (PBMT) uses light to stimulate cells. The molecular basis of the effects of PBMT is being unveiled, but it is stated that the cytochrome-c oxidase enzyme in mitochondria, a photon acceptor of PBMT, contributes to an increase in ATP production and modulates the reduction and oxidation of electron carriers NADH and FAD. Since its effects are not fully understood, PBMT is not used on tumors. Thus, it is interesting to investigate if its effects correlate to mitochondrial metabolism and if so, how it could be linked to the optical redox ratio (ORR), defined as the ratio of FAD/(NADH + FAD) fluorescences. To that end, fibroblasts (HDFn cell line) and oral squamous cell carcinoma (SCC-25 cell line) were irradiated with a light source of 780 nm and a total dose of 5 J/cm2, and imaged by optical microscopy. PBMT down-regulated the SCC-25 ORR by 10%. Furthermore, PBMT led to an increase in ROS and ATP production in carcinoma cells after 4 h, while fibroblasts only had a modest ATP increase 6 h after irradiation. Cell lines did not show distinct cell cycle profiles, as both had an increase in G2/M cells. This study indicates that PBMT decreases the redox state of oral cancer by possibly increasing glycolysis and affects normal and tumor cells through distinct pathways. To our knowledge, this is the first study that investigated the effects of PBMT on mitochondrial metabolism from the initiation of the cascade to DNA replication. This is an essential step in the investigation of the mechanism of action of PBMT in an effort to avoid misinterpretations of a variety of combined protocols.
- Subjects :
- chemical and pharmacologic phenomena
Mitochondrion
01 natural sciences
Article
010309 optics
03 medical and health sciences
0103 physical sciences
medicine
Glycolysis
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Oxidase test
Chemistry
fungi
Metabolism
Cell cycle
Atomic and Molecular Physics, and Optics
Cell biology
Enzyme
Mechanism of action
Cell culture
MITOCÔNDRIAS
Cancer cell
medicine.symptom
Biotechnology
Subjects
Details
- ISSN :
- 21567085
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Biomedical Optics Express
- Accession number :
- edsair.doi.dedup.....a9229dad63a93a893280ee26bb419f99
- Full Text :
- https://doi.org/10.1364/boe.421302