Back to Search
Start Over
Fabrication and properties of developed collagen/strontium-doped Bioglass scaffolds for bone tissue engineering
- Source :
- Journal of Materials Research and Technology, Vol 9, Iss 6, Pp 14799-14817 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- This study was conducted to evaluate the bone regeneration effects of collagen/strontium-doped Bioglass (BG-Sr) scaffolds. The present study comprised four parts including designing and fabricating scaffolds, characterization tests such as biodegradation, water uptake, porosity, FTIR (Fourier transform infrared spectroscopy), and SEM (Scanning electron microscope), in vitro tests such as MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)), alkaline phosphatase, calcium assay, Alizarin Red staining, and real-time PCR (Polymerase chain reaction), and ultimately in vivo and histopathological tests such as evaluating bone regeneration, mineralization with Hematoxylin-Eosin, Masson's trichrome and Alizarin Red staining, and histomorphometric studies 4, 8 and 12 weeks after implanting the scaffolds in the skull and femur of 36 rabbits. Significant reductions were observed in degradability, water absorption, and porosity in the BG-Sr samples. In vitro results showed that the synthetic scaffolds were less toxic, stimulated, and induced cell growth. The ALP (Alkaline phosphatase) activity was higher on day 14 than on day 7, and the highest calcium content was observed in the BG-Sr samples on day 14. In line with the SEM and MTT results, the DAPI (Diamidino-2-phenylindole) results confirmed cell proliferation. Moreover, osteocalcin and ALP gene expression were high in the BG-Sr group. The results of the histopathological tests showed high levels of bone formation in the BG-Sr scaffolds. As a scaffold in regenerative medicine, the BG-Sr composite can be an effective product for bone regeneration.
- Subjects :
- lcsh:TN1-997
Materials science
ALIZARIN RED
chemistry.chemical_element
Rabbit
02 engineering and technology
Calcium
01 natural sciences
Scaffold
Biomaterials
Trichrome
0103 physical sciences
Bioactive glass
Fourier transform infrared spectroscopy
Bone regeneration
lcsh:Mining engineering. Metallurgy
010302 applied physics
biology
Metals and Alloys
021001 nanoscience & nanotechnology
Surfaces, Coatings and Films
Staining
chemistry
Strontium
Ceramics and Composites
Osteocalcin
biology.protein
Alkaline phosphatase
0210 nano-technology
Nuclear chemistry
Subjects
Details
- ISSN :
- 22387854
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of Materials Research and Technology
- Accession number :
- edsair.doi.dedup.....a91c9f65af05479ed0c8a104ca34523f