Back to Search
Start Over
HOPE-preservation of paraffin-embedded sputum samples–A new way of bioprofiling in COPD
- Source :
- Respiratory Medicine. 107(4):587-595
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- SummaryInduced sputum is a non-invasive sampling technique for the analysis of airway inflammation in various lung diseases and comprises valuable potential for the identification of biomarkers and therapeutic targets by molecular methods. In the context of biobanking with preservation of induced sputum samples for subsequent analyses we applied the HEPES-glutamic acid buffer-mediated organic solvent protection effect (HOPE)-technique for preparation of induced sputum samples.Induced sputum samples of 20 patients with moderate to severe chronic obstructive pulmonary disease (COPD) and 12 healthy controls were collected. Cell pellets of induced sputum samples were preserved with HOPE and subsequently embedded in paraffin. Immunostaining of paraffin-block sections for interleukin-8, interleukin-17, myeloperoxidase, matrixmetalloproteinase-9, CD68, and CD8 revealed distinct signals without antigen retrieval. Moreover, RNA was extracted and successfully used for transcription microarray analysis.Sputum samples preserved by the HOPE-technique display a tool to address scientific approaches in pulmonary research, which can enable the identification of new biomarkers and therapeutic targets in respiratory diseases.
- Subjects :
- Male
Morphology
Biobanking
Pulmonary and Respiratory Medicine
Pathology
medicine.medical_specialty
Sputum Cytology
Tissue Fixation
Induced sputum
Pulmonary disease
Cell Count
Pulmonary Disease, Chronic Obstructive
chemistry.chemical_compound
Forced Expiratory Volume
Humans
Medicine
Aged
Biological Specimen Banks
Oligonucleotide Array Sequence Analysis
COPD
Paraffin Embedding
Lung
business.industry
Gene Expression Profiling
Smoking
Sputum
Middle Aged
medicine.disease
respiratory tract diseases
medicine.anatomical_structure
Antigen retrieval
chemistry
Immunology
RNA
Female
Inflammation Mediators
medicine.symptom
business
Transcriptome
HEPES
Biomarkers
Immunostaining
Immunocytochemistry
Subjects
Details
- ISSN :
- 09546111
- Volume :
- 107
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Respiratory Medicine
- Accession number :
- edsair.doi.dedup.....a90fa791fa239c8e9cc04e21148e0562
- Full Text :
- https://doi.org/10.1016/j.rmed.2012.12.006