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A plantâspecific SWR1 chromatinâremodeling complex couples histone H2A.Z deposition with nucleosome sliding
- Source :
- EMBO J
- Publication Year :
- 2020
- Publisher :
- EMBO, 2020.
-
Abstract
- Deposition of H2A.Z in chromatin is known to be mediated by a conserved SWR1 chromatin-remodeling complex in eukaryotes. However, little is known about whether and how the SWR1 complex cooperates with other chromatin regulators. Using immunoprecipitation followed by mass spectrometry, we found all known components of the Arabidopsis thaliana SWR1 complex and additionally identified the following three classes of previously uncharacterized plant-specific SWR1 components: MBD9, a methyl-CpG-binding domain-containing protein; CHR11 and CHR17 (CHR11/17), ISWI chromatin remodelers responsible for nucleosome sliding; and TRA1a and TRA1b, accessory subunits of the conserved NuA4 histone acetyltransferase complex. MBD9 directly interacts with CHR11/17 and the SWR1 catalytic subunit PIE1, and is responsible for the association of CHR11/17 with the SWR1 complex. MBD9, TRA1a, and TRA1b function as canonical components of the SWR1 complex to mediate H2A.Z deposition. CHR11/17 are not only responsible for nucleosome sliding but also involved in H2A.Z deposition. These results indicate that the association of the SWR1 complex with CHR11/17 may facilitate the coupling of H2A.Z deposition with nucleosome sliding, thereby co-regulating gene expression, development, and flowering time.
- Subjects :
- Immunoprecipitation
Protein subunit
Arabidopsis
Biology
General Biochemistry, Genetics and Molecular Biology
Chromatin remodeling
Histones
03 medical and health sciences
0302 clinical medicine
Gene expression
Arabidopsis thaliana
Protein Interaction Maps
NuA4 histone acetyltransferase complex
Molecular Biology
Histone Acetyltransferases
030304 developmental biology
Adenosine Triphosphatases
0303 health sciences
General Immunology and Microbiology
Arabidopsis Proteins
General Neuroscience
Articles
Chromatin Assembly and Disassembly
biology.organism_classification
Chromatin
Swr1 complex
Nucleosomes
Cell biology
DNA-Binding Proteins
030217 neurology & neurosurgery
Transcription Factors
Subjects
Details
- ISSN :
- 14602075 and 02614189
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....a8f59d853ffda5376d622c184cb26c4c