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Comprehensive NOE characterization of a partially folded large fragment of staphylococcal nuclease Delta131Delta, using NMR methods with improved resolution
- Source :
- Journal of molecular biology. 272(1)
- Publication Year :
- 1997
-
Abstract
- Comprehensive NOE results from detailed structural characterization of a 131 residue partially folded fragment of staphylococcal nuclease (Delta131Delta) made possible by NMR methods with improved resolution are presented. The resulting NOE patterns reflect sampling of both alpha and beta regions of phi, phi conformational space, yet demonstrate significant preferences for both native-like and non-native-like turn and potentially helical conformations. Together with data from studies of the unfolded state of the drkN SH3 domain, NOE patterns observed for partially folded or unfolded proteins are summarized. It is surprising that few long-range NOEs were observed in Delta131Delta. The two longest-range NOEs are both native-like; one of these, an (i,i+5) NOE, provides evidence for a Schellman capping motif for helix termination. Many aliphatic-aliphatic and aliphatic-amide NOEs, which are not normally observed in folded proteins, were detected. We have ruled out significant contributions from spin-diffusion for a number of these NOEs and suggest that one source may be sampling of non-prolyl cis peptide bond configurations in the disordered state of Delta131Delta.
- Subjects :
- inorganic chemicals
Protein Folding
Magnetic Resonance Spectroscopy
biology
Chemistry
Protein Conformation
Molecular Sequence Data
Nuclear magnetic resonance spectroscopy
SH3 domain
Peptide Fragments
Crystallography
Protein structure
Structural Biology
biology.protein
Peptide bond
Micrococcal Nuclease
Protein folding
Amino Acid Sequence
Molecular Biology
Two-dimensional nuclear magnetic resonance spectroscopy
Heteronuclear single quantum coherence spectroscopy
Micrococcal nuclease
Subjects
Details
- ISSN :
- 00222836
- Volume :
- 272
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of molecular biology
- Accession number :
- edsair.doi.dedup.....a8ed3a3fb4d3f0f6c8a3e8553e2c1fdf