Supplementary Figure 4 from Coordinate Hyperactivation of Notch1 and Ras/MAPK Pathways Correlates with Poor Patient Survival: Novel Therapeutic Strategy for Aggressive Breast Cancers

Supplementary Figure 4: Breast cancer cell lines were subjected to sphere formation in the presence of anti-Notch1 MAb602.101 (10microg/ml), 10microM MAPK inhibitor (PD98059), combination of MAb602.101 and PD98059 and appropriate controls (IgG and DMSO). After a week cells were dissociated and subjected for secondary sphere formation in the absence of any treatments. Photomicrographs represent phase contrast images of secondary spheres formed by MDA-MB-231 and BT-474 cells; magnification 10x.