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Knockout rats via embryo microinjection of zinc-finger nucleases
- Source :
- Science (New York, N.Y.). 325(5939)
- Publication Year :
- 2009
-
Abstract
- The toolbox of rat genetics currently lacks the ability to introduce site-directed, heritable mutations into the genome to create knockout animals. By using engineered zinc-finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one-cell rat embryo leads to a high frequency of animals carrying 25 to 100% disruption at the target locus. These mutations are faithfully and efficiently transmitted through the germline. Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.
- Subjects :
- Male
Knockout rat
Microinjections
Green Fluorescent Proteins
Molecular Sequence Data
Biology
Germline
Article
chemistry.chemical_compound
Gene Knockout Techniques
Animals
RNA, Messenger
Gene
Multidisciplinary
Endodeoxyribonucleases
Base Sequence
RNA
Embryo
Zinc Fingers
DNA
Embryo, Mammalian
Zinc finger nuclease
Molecular biology
Rats
chemistry
Immunoglobulin M
rab GTP-Binding Proteins
Mutagenesis, Site-Directed
Feasibility Studies
Female
Subjects
Details
- ISSN :
- 10959203
- Volume :
- 325
- Issue :
- 5939
- Database :
- OpenAIRE
- Journal :
- Science (New York, N.Y.)
- Accession number :
- edsair.doi.dedup.....a8dfde293036e526492b11e92ebd5362