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In vivo third-harmonic generation microscopy study on vitiligo patients
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2019
- Publisher :
- Society of Photo-Optical Instrumentation Engineers, 2019.
-
Abstract
- Melanin is known to provide strong third-harmonic generation (THG) contrast in human skin. With a high concentration in basal cell cytoplasm, THG contrast provided by melanin overshadows other THG sources in human skin studies. For better understanding of the THG signals in keratinocytes without the influence of melanin, an in vivo THG microscopy (THGM) study was first conducted on vitiliginous skin. As a result, the THG-brightness ratio between the melanin-lacking cytoplasm of basal cells and collagen fibers is about 1.106 at the dermal–epidermal junctions of vitiliginous skin, indicating high sensitivity of THGM for the presence of melanin. We further applied the in vivo THGM to assist evaluating the therapeutic outcome from the histopathological point of view for those showed no improvement under narrowband ultraviolet B therapy based on the seven-point Physician Global Assessment score. Our clinical study indicates the high potential of THGM to assist the histopathological assessment of the therapeutic efficacy of vitiligo treatments.
- Subjects :
- Paper
vitiligo
Chromium
Keratinocytes
Pathology
medicine.medical_specialty
skin
Optical Phenomena
Biomedical Engineering
Human skin
Vitiligo
01 natural sciences
010309 optics
Biomaterials
Melanin
third-harmonic generation
Basal (phylogenetics)
Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
In vivo
0103 physical sciences
Medicine
Humans
Third Harmonic Generation Microscopy
Melanins
integumentary system
business.industry
Lasers
Silicon Compounds
basal keratinocytes
Equipment Design
medicine.disease
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
melanin
melanocytes
Cytoplasm
Second Harmonic Generation Microscopy
Ultraviolet Therapy
sense organs
Collagen
business
Preclinical imaging
harmonic generation microscopy
Subjects
Details
- Language :
- English
- ISSN :
- 15602281 and 10833668
- Volume :
- 25
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....a8c77f17efd98a3ee261d284eb55026f