Back to Search
Start Over
Effects of a new topic amikacin formulation on chemotaxis and release of profibrotic factors by human monocytes
- Publication Year :
- 1998
-
Abstract
- Aminoglycosides, widely used because of their large-spectrum antibiotic effects, should not interfere with the healing process of an ulcer or an infected wound. We evaluated the effects of amikacin or the excipients present in the topic formulation BG 90, powder 2.5% (Boniscontro e Gazzone S.r.l., Rome, Italy), on human monocyte chemotaxis and the release of profibrotic factors by resting or lipopolysaccharide (LPS)-activated monocytes. The chemotactic response of monocytes to zymosan-activated serum is not modified in vitro by pre-incubation of the cells with amikacin (2 and 10 µg/ml/106 cells) or excipients. Unstimulated monocytes did not secrete appreciable amounts of cytokines. Vice versa, amikacin-stimulated cells released platelet-derived growth factor AB (PDGF-AB) (about 340 pg/ml), transforming growth factor (TGF)-β1 (about 10 pg/ml), and tumour necrosis factor (TNF)-α (over 1,100 pg/ml); among excipients, ZnO and vitamin E induced PDGF-AB release (about 320 and, respectively, 200 pg/ml), while stimulation of monocyte monolayers by the other excipients did not lead to appreciable cytokine release. As expected, LPS-activated human monocytes produced PDGF-AB, TGF-β1, and TNF-α. When monocytes were co-stimulated with LPS and amikacin, the PDGF-AB and TGF-β1 values almost overlapped with those from the stimulation of cells with LPS alone, while TNF-α production was slowly reduced. The results show a stimulating effect of aminoglycoside on the production of profibrotic factors and, therefore, on the healing process of wounds in addition to a modulating effect on the production of pro-inflammatory cytokines like TNF-α. Moreover, ZnO and tocopherol (free-radical scavengers), used as excipients in the topic formulation, induce the release of growth factors with profibrotic activity (PDGF-AB). Further research is warranted to explore the effects of this formulation in vivo, verifying whether the association of the antibiotic with scavengers has a double advantage in topical amikacin: on the one hand, it could limit the damage from free radicals, and on the other it could favour tissue healing.
- Subjects :
- Lipopolysaccharides
Lipopolysaccharide
Monocyte chemotaxis
medicine.medical_treatment
Administration, Topical
Chemistry, Pharmaceutical
Pharmacology
Biology
Monocytes
Microbiology
chemistry.chemical_compound
In vivo
Transforming Growth Factor beta
Drug Discovery
medicine
Humans
Pharmacology (medical)
Amikacin
Antibacterial agent
Platelet-Derived Growth Factor
Tumor Necrosis Factor-alpha
Monocyte
Growth factor
General Medicine
Anti-Bacterial Agents
Chemotaxis, Leukocyte
Infectious Diseases
Cytokine
medicine.anatomical_structure
Oncology
chemistry
Cytokines
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a8c2194e8071bcaab99eae9a0e4353cc