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Conjugated linoleic acid improves endothelial Ca2+ signaling by blocking growth factor and cytokine-mediated Cx43 phosphorylation
- Source :
- Mol Cell Endocrinol
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Sustained Ca2+ burst signaling is crucial for endothelial vasodilator production and is disrupted by growth factors and cytokines. Conjugated linoleic acid (CLA), a Src inhibitor in certain preparations, is generally regarded as safe during pregnancy by the FDA. Multiple CLA preparations; t10, c12 or c9, t11 CLA, or a 1:1 mixture of the two were administered before growth factor or cytokine treatment. Growth factors and cytokines caused a significant decrease in Ca2+ burst numbers in response to ATP stimulation. Both t10, c12 CLA and the 1:1 mixture rescued VEGF165 or TNFα inhibited Ca2+ bursts and correlated with Src-specific phosphorylation of connexin 43. VEGF165, TNFα, and IL-6 in combination at physiologic concentrations revealed IL-6 amplified the inhibitory effects of lower dose of VEGF165 and TNFα. Again, the 1:1 CLA mixture was most effective at rescue of function. Therefore, CLA formulations may be a promising treatment for endothelial dysfunction in diseases such as preeclampsia.
- Subjects :
- Vascular Endothelial Growth Factor A
0301 basic medicine
medicine.medical_treatment
Conjugated linoleic acid
Connexin
030209 endocrinology & metabolism
Stimulation
Pharmacology
Biochemistry
Article
03 medical and health sciences
chemistry.chemical_compound
Adenosine Triphosphate
0302 clinical medicine
Endocrinology
Isomerism
Human Umbilical Vein Endothelial Cells
medicine
Humans
Linoleic Acids, Conjugated
Calcium Signaling
Phosphorylation
Endothelial dysfunction
Phosphotyrosine
Molecular Biology
integumentary system
Tumor Necrosis Factor-alpha
Chemistry
Growth factor
food and beverages
medicine.disease
030104 developmental biology
Cytokine
Connexin 43
Cytokines
Intercellular Signaling Peptides and Proteins
Regression Analysis
Fibroblast Growth Factor 2
lipids (amino acids, peptides, and proteins)
Tumor necrosis factor alpha
Subjects
Details
- ISSN :
- 03037207
- Volume :
- 510
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Endocrinology
- Accession number :
- edsair.doi.dedup.....a89d31e783ba38fcad33ac7c816e1c2a