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Equine Herpesvirus-2 E10 Gene Product, but Not Its Cellular Homologue, Activates NF-κB Transcription Factor and c-Jun N-terminal Kinase
- Source :
- Journal of Biological Chemistry, vol. 274, no. 15, pp. 9962-9968
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- We have previously reported on the death effector domain containing E8 gene product from equine herpesvirus-2, designated FLICE inhibitory protein (v-FLIP), and on its cellular homologue, c-FLIP, which inhibit the activation of caspase-8 by death receptors. Here we report on the structure and function of the E10 gene product of equine herpesvirus-2, designated v-CARMEN, and on its cellular homologue, c-CARMEN, which contain a caspase-recruiting domain (CARD) motif. c-CARMEN is highly homologous to the viral protein in its N-terminal CARD motif but differs in its C-terminal extension. v-CARMEN and c-CARMEN interact directly in a CARD-dependent manner yet reveal different binding specificities toward members of the tumor necrosis factor receptor-associated factor (TRAF) family. v-CARMEN binds to TRAF6 and weakly to TRAF3 and, upon overexpression, potently induces the c-Jun N-terminal kinase (JNK), p38, and nuclear factor (NF)-kappaB transcriptional pathways. c-CARMEN or truncated versions thereof do not appear to induce JNK and NF-kappaB activation by themselves, nor do they affect the JNK and NF-kappaB activating potential of v-CARMEN. Thus, in contrast to the cellular homologue, v-CARMEN may have additional properties in its unique C terminus that allow for an autonomous activator effect on NF-kappaB and JNK. Through activation of NF-kappaB, v-CARMEN may regulate the expression of the cellular and viral genes important for viral replication.
- Subjects :
- TRAF3
Viral protein
Molecular Sequence Data
CASP8 and FADD-Like Apoptosis Regulating Protein
Apoptosis
Biology
medicine.disease_cause
p38 Mitogen-Activated Protein Kinases
Biochemistry
Receptors, Tumor Necrosis Factor
Gene product
Jurkat Cells
Mice
Viral Proteins
medicine
Animals
Humans
Amino Acid Sequence
Molecular Biology
Transcription factor
Adaptor Proteins, Signal Transducing
Sequence Homology, Amino Acid
Activator (genetics)
Kinase
c-jun
Intracellular Signaling Peptides and Proteins
JNK Mitogen-Activated Protein Kinases
NF-kappa B
Cell Biology
B-Cell CLL-Lymphoma 10 Protein
Molecular biology
Neoplasm Proteins
Enzyme Activation
Caspases
Calcium-Calmodulin-Dependent Protein Kinases/metabolism
Carrier Proteins/metabolism
Caspases/metabolism
Mitogen-Activated Protein Kinases
NF-kappa B/metabolism
Receptors, Tumor Necrosis Factor/metabolism
Viral Proteins/metabolism
Calcium-Calmodulin-Dependent Protein Kinases
Death effector domain
Carrier Proteins
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 274
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....a88db9e2e2dfa3a356b095ae215ec48e