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Survival of adult generated hippocampal neurons is altered in circadian arrhythmic mice
- Source :
- PLoS ONE, Vol 9, Iss 6, p e99527 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- The subgranular zone of the hippocampal formation gives rise to new neurons that populate the dentate gyrus throughout life. Cells in the hippocampus exhibit rhythmic clock gene expression and the circadian clock is known to regulate the cycle of cell division in other areas of the body. These facts suggest that the circadian clock may regulate adult neurogenesis in the hippocampus as well. In the present study, neurogenesis in the hippocampal subgranular zone was examined in arrhythmic Bmal1 knockout (-KO) mice and their rhythmic heterozygous and wildtype littermates. Proliferation and survival of newly generated subgranular zone cells were examined using bromodeoxyuridine labelling, while pyknosis (a measure of cell death) and hippocampal volume were examined in cresyl violet stained sections. There was no significant difference in cellular proliferation between any of the groups, yet survival of proliferating cells, 6 weeks after the bromodeoxyuridine injection, was significantly greater in the BMAL1-KO animals. The number of pyknotic cells was significantly decreased in Bmal1-KO animals, yet hippocampal volume remained the same across genotypes. These findings suggest that while a functional circadian clock is not necessary for normal proliferation of neuronal precursor cells, the normal pruning of newly generated neurons in the hippocampus may require a functional circadian clock.
- Subjects :
- Aging
Heterozygote
medicine.medical_specialty
Cell Survival
Neurogenesis
Circadian clock
Hippocampus
lcsh:Medicine
Biology
Hippocampal formation
Biochemistry
Subgranular zone
Developmental Neuroscience
Internal medicine
Hippocampal Neurogenesis
medicine
Animals
Circadian rhythm
lcsh:Science
Cell Proliferation
Mice, Knockout
Neurons
Multidisciplinary
Behavior, Animal
Dentate gyrus
Homozygote
lcsh:R
Adult Neurogenesis
ARNTL Transcription Factors
Biology and Life Sciences
Organ Size
Circadian Rhythm
CLOCK
Circadian Oscillators
Circadian Rhythms
Endocrinology
medicine.anatomical_structure
nervous system
Cellular Neuroscience
Dentate Gyrus
lcsh:Q
Chronobiology
Research Article
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a88960d39bfa3d827905c3fde2ecbcd8