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Expression of retinaldehyde dehydrogenase (RALDH)2 and RALDH3 but not RALDH1 in the developing anterior pituitary glands of rats
- Source :
- Cell and tissue research. 328(1)
- Publication Year :
- 2006
-
Abstract
- Retinoic acid (RA) plays an important role in cell growth and tissue development and is also a regulating factor of pituitary function. However, whether RA is generated in the pituitary gland and plays a role as a paracrine and/or autocrine hormone is generally unknown. RA is synthesized from retinoids through oxidation processes. Dehydrogenases catalyzing the oxidation of retinal to RA are members of the retinaldehyde dehydrogenase (RALDH) family. In this study, we examined the expression of RALDH1, RALDH2, and RALDH3 mRNA in the rat embryonic pituitary gland. By in situ hybridization with digoxigenin-labeled cRNA probes, we detected mRNA expression for RALDH2 and RALDH3, but not RALDH1. The expression of RALDH2 and RALDH3 was located in Rathke’s pouch at embryonic day 12.5 (E12.5) and subsequently in the developing anterior pituitary gland. We also used quantitative real-time polymerase chain reaction to analyze RALDH2 and RALDH3 mRNA expression levels during the development of the pituitary gland. We found that pituitary RALDH2 and RALDH3 mRNA levels were high at E17.5 and decreased markedly after birth. Our study is the first to show that RALDH2 and RALDH3, but not RALDH1, are expressed in the embryonic anterior pituitary gland of the rat.
- Subjects :
- Pituitary gland
medicine.medical_specialty
Histology
Retinoic acid
In situ hybridization
Biology
Aldehyde Dehydrogenase 1 Family
Gene Expression Regulation, Enzymologic
Pathology and Forensic Medicine
Paracrine signalling
chemistry.chemical_compound
Anterior pituitary
Pituitary Gland, Anterior
Internal medicine
medicine
Animals
RNA, Messenger
Rats, Wistar
Autocrine signalling
Messenger RNA
Gene Expression Regulation, Developmental
Retinal Dehydrogenase
Retinal
Cell Biology
Aldehyde Oxidoreductases
Rats
medicine.anatomical_structure
Endocrinology
chemistry
embryonic structures
Subjects
Details
- ISSN :
- 0302766X
- Volume :
- 328
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cell and tissue research
- Accession number :
- edsair.doi.dedup.....a884ac618831f64d2ef01d0c8ecafec0