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Molecular and Antigenic Characterization of Reassortant H3N2 Viruses from Turkeys with a Unique Constellation of Pandemic H1N1 Internal Genes
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 3, p e32858 (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science, 2012.
-
Abstract
- Triple reassortant (TR) H3N2 influenza viruses cause varying degrees of loss in egg production in breeder turkeys. In this study we characterized TR H3N2 viruses isolated from three breeder turkey farms diagnosed with a drop in egg production. The eight gene segments of the virus isolated from the first case submission (FAV-003) were all of TR H3N2 lineage. However, viruses from the two subsequent case submissions (FAV-009 and FAV-010) were unique reassortants with PB2, PA, nucleoprotein (NP) and matrix (M) gene segments from 2009 pandemic H1N1 and the remaining gene segments from TR H3N2. Phylogenetic analysis of the HA and NA genes placed the 3 virus isolates in 2 separate clades within cluster IV of TR H3N2 viruses. Birds from the latter two affected farms had been vaccinated with a H3N4 oil emulsion vaccine prior to the outbreak. The HAl subunit of the H3N4 vaccine strain had only a predicted amino acid identity of 79% with the isolate from FAV-003 and 80% for the isolates from FAV-009 and FAV-0010. By comparison, the predicted amino acid sequence identity between a prototype TR H3N2 cluster IV virus A/Sw/ON/33853/2005 and the three turkey isolates from this study was 95% while the identity between FAV-003 and FAV-009/10 isolates was 91%. When the previously identified antigenic sites A, B, C, D and E of HA1 were examined, isolates from FAV-003 and FAV-009/10 had a total of 19 and 16 amino acid substitutions respectively when compared with the H3N4 vaccine strain. These changes corresponded with the failure of the sera collected from turkeys that received this vaccine to neutralize any of the above three isolates in vitro.
- Subjects :
- Veterinary Medicine
Turkeys
Genotype
viruses
Molecular Sequence Data
lcsh:Medicine
Biology
medicine.disease_cause
Virus
Antibodies
Influenza A Virus, H1N1 Subtype
Zoonoses
Influenza A virus
medicine
Genetics
Animals
Cluster Analysis
Amino Acid Sequence
lcsh:Science
Gene
Peptide sequence
Pandemics
Phylogeny
Poultry Diseases
Multidisciplinary
Zoonotic Diseases
Viral Vaccine
Influenza A Virus, H3N2 Subtype
lcsh:R
Genomics
Virology
Nucleoprotein
Protein Structure, Tertiary
Infectious Diseases
Hemagglutinins
Veterinary Diseases
Influenza Vaccines
Influenza in Birds
biology.protein
Medicine
lcsh:Q
Veterinary Science
Antibody
Sequence Alignment
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a87f541d73d9f0912763050f8b16957c