Back to Search
Start Over
CPAP is a cell-cycle regulated protein that controls centriole length
- Source :
- Nature Cell Biology. 11:825-831
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Centriole duplication involves the growing of a procentriole (progeny centriole) next to the proximal end of each pre-existing centriole (parental centriole). The molecular mechanisms that regulate procentriole elongation remain obscure. We show here that expression of the centriolar protein CPAP (centrosomal P4.1-associated protein) is carefully regulated during the cell cycle, with the protein being degraded in late mitosis. Depletion of CPAP inhibited centrosome duplication, whereas excess CPAP induced the formation of elongated procentriole-like structures (PLSs), which contain stable microtubules and several centriolar proteins. Ultrastructural analysis revealed that these structures are similar to procentrioles with elongated microtubules. Overexpression of a CPAP mutant (CPAP-377EE) that does not bind to tubulin dimers significantly inhibited the formation of CPAP-induced PLSs. Together, these results suggest that CPAP is a new regulator of centriole length and its intrinsic tubulin-dimer binding activity is required for procentriole elongation.
- Subjects :
- PLK4
Centriole
sports
Immunoblotting
Mitosis
Cell Cycle Proteins
Biology
Models, Biological
Centriole elongation
Cell Line
Procentriole
Microscopy, Electron, Transmission
Tubulin
Cell Line, Tumor
Humans
Immunoprecipitation
Basal body
RNA, Small Interfering
Centrioles
Microscopy, Confocal
Cell Cycle
Ubiquitination
Cell Biology
Cell cycle
Flow Cytometry
Cell biology
sports.league
Microscopy, Fluorescence
CEP135
Microtubule-Associated Proteins
HeLa Cells
Centriole assembly
Subjects
Details
- ISSN :
- 14764679 and 14657392
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Nature Cell Biology
- Accession number :
- edsair.doi.dedup.....a8770bede1a4e972e8db33dcdd6932f7