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Transcriptomic survey reveals multiple adaptation mechanisms in response to nitrogen deprivation in marine Porphyridium cruentum
- Source :
- PLoS ONE, Vol 16, Iss 11 (2021), PLoS ONE, Vol 16, Iss 11, p e0259833 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Single-cell red microalga Porphyridium cruentum is potentially considered to be the bioresource for biofuel and pharmaceutical production. Nitrogen is a kind of nutrient component for photosynthetic P. cruentum. Meanwhile, nitrogen stress could induce to accumulate some substances such as lipid and phycoerythrin and affect its growth and physiology. However, how marine microalga Porphyridium cruentum respond and adapt to nitrogen starvation remains elusive. Here, acclimation of the metabolic reprogramming to changes in the nutrient environment was studied by high-throughput mRNA sequencing in the unicellular red alga P. cruentum. Firstly, to reveal transcriptional regulation, de novo transcriptome was assembled and 8,244 unigenes were annotated based on different database. Secondly, under nitrogen deprivation, 2100 unigenes displayed differential expression (1134 upregulation and 966 downregulation, respectively) and some pathways including carbon/nitrogen metabolism, photosynthesis, and lipid metabolism would be reprogrammed in P. cruentum. The result demonstrated that nitrate assimilation (with related unigenes of 8–493 fold upregulation) would be strengthen and photosynthesis (with related unigenes of 6–35 fold downregulation) be impaired under nitrogen deprivation. Importantly, compared to other green algae, red microalga P. cruentum presented a different expression pattern of lipid metabolism in response to nitrogen stress. These observations will also provide novel insight for understanding adaption mechanisms and potential targets for metabolic engineering and synthetic biology in P. cruentum.
- Subjects :
- Nitrogen assimilation
Nitrogen Metabolism
Gene Expression
Plant Science
Biochemistry
Database and Informatics Methods
RNA-Seq
Photosynthesis
Multidisciplinary
biology
Plant Biochemistry
Chemistry
Eukaryota
Genomics
Plants
Lipids
Adaptation, Physiological
Physical Sciences
Medicine
Transcriptome Analysis
Sequence Analysis
Research Article
Algae
Bioinformatics
Nitrogen
Science
Sequence Databases
Research and Analysis Methods
Real-Time Polymerase Chain Reaction
Metabolic engineering
Genetics
Nitrogen cycle
Nitrates
Organisms
Chemical Compounds
Biology and Life Sciences
Computational Biology
Lipid metabolism
Genome Analysis
Lipid Metabolism
biology.organism_classification
Metabolic pathway
Metabolism
Biological Databases
MRNA Sequencing
Gene Expression Regulation
Porphyridium cruentum
Porphyridium
Transcriptome
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....a85ecb1caa0970bb78acd47211425009