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A highly optimized DNA vaccine confers complete protective immunity against high-dose lethal lymphocytic choriomeningitis virus challenge
- Publication Year :
- 2011
-
Abstract
- Protection against infection is the hallmark of immunity and the basis of effective vaccination. For a variety of reasons there is a great demand to develop new, safer and more effective vaccine platforms. In this regard, while ‘first-generation’ DNA vaccines were poorly immunogenic, new genetic ‘optimization’ strategies and the application of in vivo electroporation (EP) have dramatically boosted their potency. We developed a highly optimized plasmid DNA vaccine that expresses the lymphocytic choriomeningitis virus (LCMV) nucleocapsid protein (NP) and evaluated it using the LCMV challenge model, a gold standard for studying infection and immunity. When administered intramuscularly with EP, robust NP-specific cellular and humoral immune responses were elicited, the magnitudes of which approached those following acute LCMV infection. Furthermore, these responses were capable of providing 100% protection against a high-dose, normally lethal virus challenge. This is the first non-infectious vaccine conferring complete protective immunity up to eight weeks after vaccination and demonstrates the potential utility of ‘next-generation’ DNA vaccines.
- Subjects :
- Enzyme-Linked Immunospot Assay
Genetic Vectors
chemical and pharmacologic phenomena
Biology
Lymphocytic Choriomeningitis
Lymphocytic choriomeningitis
Transfection
Median lethal dose
Virus
Article
DNA vaccination
Lethal Dose 50
Mice
Immune system
Immunity
medicine
Vaccines, DNA
Animals
Humans
Lymphocytic choriomeningitis virus
Immunity, Cellular
General Veterinary
General Immunology and Microbiology
Viral Vaccine
Vaccination
Public Health, Environmental and Occupational Health
Viral Vaccines
Nucleocapsid Proteins
medicine.disease
Virology
Immunity, Humoral
Mice, Inbred C57BL
Infectious Diseases
HEK293 Cells
Immunology
Antibody Formation
Molecular Medicine
Female
Plasmids
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a8578c20a53cd1b923755e01b56bcb05