Back to Search
Start Over
Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing
- Source :
- BMC Musculoskeletal Disorders
- Publication Year :
- 2015
-
Abstract
- Background The recent discovery of residing tendon stem/progenitor cells has triggered a growing interest in stem cells as a useful tool in tendon repair. Our knowledge of their involvement in naturally healing tendons is, however, sparse. The aim of this study was to identify and determine stem/progenitor cells in relation to different healing phases and regions in a rat model of Achilles tendon rupture. Methods Surgery was performed to create a mid-tendon rupture on the right Achilles tendon of 24 rats, whereas the left tendon was used as a control. Tendons were harvested at one, two, eight and 17 weeks post-rupture and stained with antibodies specific to stem/progenitor cells (Octamer-binding transcription factor 3/4 (Oct 3/4) and nucleostemin), migrating cells (Dynamin 2 (Dyn 2)) and leukocytes (CD45). A histological examination was performed on sections stained with Alcian blue. Results At one and two weeks post-rupture, a large number of stem/progenitor cells were discovered throughout the tendon. Most of these cells were nucleostemin positive, whereas only a few Oct 3/4-positive cells were found, mainly situated inside the injury region (I region). At eight and 17 weeks, the increment in stem/progenitor cells had diminished to equal that in the control tendons. At all time points, Oct 3/4-positive cells were also found in the connective tissue surrounding the tendon and at the muscle-tendon junction in both ruptured and control tendons and were often seen at the same location as the migration marker, Dyn 2. Conclusions The whole length of the Achilles tendon is infiltrated by stem/progenitor cells at early time points after a mid-tendon rupture. However, different stem/progenitor cell populations exhibit varying anatomical and temporal expressions during Achilles tendon healing, suggesting distinct reparative implications. Oct 3/4 may thus act as a more local, migrating stem/progenitor cell involved in injury-site-specific regenerative effects, as compared to the more general proliferative role of nucleostemin-positive stem/progenitor cells.
- Subjects :
- musculoskeletal diseases
medicine.medical_specialty
Pathology
Time Factors
Octamer Transcription Factor-3
Rat model
Cell Count
Achilles Tendon
Rats, Sprague-Dawley
Dynamin II
Rheumatology
GTP-Binding Proteins
Leukocytes
Medicine
Animals
Orthopedics and Sports Medicine
Cell Lineage
Progenitor cell
Rupture
Achilles tendon
Wound Healing
business.industry
Stem Cells
Nuclear Proteins
musculoskeletal system
Tendon
Surgery
Rats
medicine.anatomical_structure
Leukocyte Common Antigens
Female
Stem cell
Achilles tendon rupture
medicine.symptom
Wound healing
business
Carrier Proteins
Research Article
Subjects
Details
- ISSN :
- 14712474
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- BMC musculoskeletal disorders
- Accession number :
- edsair.doi.dedup.....a853e5897f9b907a0a8c6e540873d699