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Chemotactic factors released from hepatocytes exposed to acetaminophen
- Source :
- Digestive Diseases and Sciences. 40:1831-1836
- Publication Year :
- 1995
- Publisher :
- Springer Science and Business Media LLC, 1995.
-
Abstract
- To clarify the mechanism of neutrophil infiltration in the liver of acetaminophen-induced hepatic injury, chemotactic factor released from hepatocytes exposed to acetaminophen has been investigated. Hepatocytes exposed to acetaminophen release nondialyzable chemotactic factor, although acetaminophen in itself inhibits chemotaxis of neutrophils. Chemotactic activity of the nondialyzable chemotactic factor was reduced after treatment with heat (56 degrees C, 30 min) or trypsin. Chemotactic activity was demonstrated at the molecular weights of around 25 and 55 kDa. Chemotactic activity of the conditioned medium was not significantly reduced in the presence of antibody against rat KC/gro protein (interleukin-8-related cytokine in rodent). Chemotactic activity of a 25-kDa factor was reduced by the antibody against the antibody against KC/gro protein, but that of a 55-kDa factor was not reduced. Immunoblot analysis revealed that the peptide reacted with antibody against rat KC/gro protein was demonstrated at a molecular weight of around 20-25 kDa, but not around 55kDa, when the conditioned medium of acetaminophen-treated hepatocytes was electrophoresed. These results suggest that hepatocytes exposed to acetaminophen release two types of chemotactic factors for neutrophils and that a major part of the chemotactic factor could be different from a member of interleukin-8 family.
- Subjects :
- Male
medicine.medical_specialty
Chemokine
Neutrophils
Physiology
Liver cytology
Chemokine CXCL1
Neutrophile
medicine.medical_treatment
Immunoblotting
Biology
Antibodies
Rats, Sprague-Dawley
Internal medicine
medicine
Animals
Growth Substances
Cells, Cultured
Acetaminophen
Chemotactic Factors
Gastroenterology
Chemotaxis
Trypsin
Rats
Molecular Weight
Chemotaxis, Leukocyte
medicine.anatomical_structure
Endocrinology
Cytokine
Liver
Hepatocyte
biology.protein
Intercellular Signaling Peptides and Proteins
Antibody
Chemokines, CXC
medicine.drug
Subjects
Details
- ISSN :
- 15732568 and 01632116
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Digestive Diseases and Sciences
- Accession number :
- edsair.doi.dedup.....a83d0c57bea7a61b2203da176f0540a6