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Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
- Source :
- Physiological Reports 3 (4), 11.p. (2016), Physiological Reports, Physiological Reports, Wiley, 2016, 4 (3), 11.p. ⟨10.14814/phy2.12686⟩, Physiological Reports, 2016, 4 (3), 11.p. ⟨10.14814/phy2.12686⟩
- Publication Year :
- 2016
-
Abstract
- This work was supported by the French National Institute for Health and Medical Research (INSERM), and by the Joseph-Fourier UniversityWe are grateful to Emily Tubbs for language corrections in this paper; The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11‐ and 14‐week‐old ZDF (fa/fa) and ZDF lean (fa/+) rats. Oxygen consumption, H2O2 release, calcium retention capacity (CRC), membrane potential, membrane fluidity, and fatty acid composition were analyzed. State 3 oxygen consumption with palmitoyl‐carnitine increases between 11 and 14 weeks of age in lean but not in diabetic animals. This response was not seen with other substrates, suggesting that the use of fatty acids is impaired in diabetic rats. H2O2 release was lower in 14‐week‐old ZDF (fa/fa) rats as compared to ZDF lean (fa/+). These changes were not associated with differences in enzymatic activities of the respiratory complexes, suggesting regulatory mechanisms independent of their expression levels. Membrane fluidity and composition analyses show only slight effects linked to diabetes progression. The most salient feature was a reduction in CRC in the presence of CsA, an effect reflecting PTP dysregulation. Our data suggest few changes of mitochondrial function in ZDF fa/fa rats. At the age of 11 weeks, liver mitochondria have mainly a reduced effect of CsA on CRC.
- Subjects :
- Male
0301 basic medicine
Magnetic Resonance Spectroscopy
endocrine system diseases
Physiology
Mitochondrion
medicine.disease_cause
oxidative
Membrane Physiology
Membrane fluidity
rat
Original Research
Membrane Potential, Mitochondrial
chemistry.chemical_classification
medicine.diagnostic_test
diabetes
acide gras
phosphorylation
Flow Cytometry
foie
mitochondria
Liver
Alimentation et Nutrition
Endocrine and Metabolic Conditons, Disorders and Treatments
diabète
medicine.medical_specialty
Blotting, Western
oxidative phosphorylation
Oxidative phosphorylation
Diabetes Mellitus, Experimental
respiration mitochondriale
03 medical and health sciences
Oxygen Consumption
Physiology (medical)
Diabetes mellitus
Internal medicine
ZDF
Metabolism and Regulation
medicine
Animals
Food and Nutrition
Reactive oxygen species
Electron Spin Resonance Spectroscopy
Fatty acid
nutritional and metabolic diseases
fonction mitochondriale
medicine.disease
Rats
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
Oxidative Stress
030104 developmental biology
Endocrinology
Diabetes Mellitus, Type 2
chemistry
liver function tests
fatty acid
Reactive Oxygen Species
Liver function tests
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 2051817X
- Database :
- OpenAIRE
- Journal :
- Physiological Reports 3 (4), 11.p. (2016), Physiological Reports, Physiological Reports, Wiley, 2016, 4 (3), 11.p. ⟨10.14814/phy2.12686⟩, Physiological Reports, 2016, 4 (3), 11.p. ⟨10.14814/phy2.12686⟩
- Accession number :
- edsair.doi.dedup.....a834ea485afa748c8e23f4dcd196d5e8