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Proteomic and Transcriptomic Patterns during Lipid Remodeling in Nannochloropsis gaditana
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 18, International Journal of Molecular Sciences, Vol 21, Iss 6946, p 6946 (2020), International Journal of Molecular Sciences 21 (2020) 18, International Journal of Molecular Sciences, 21(18)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Nutrient limited conditions are common in natural phytoplankton communities and are often used to increase the yield of lipids from industrial microalgae cultivations. Here we studied the effects of bioavailable nitrogen (N) and phosphorus (P) deprivation on the proteome and transcriptome of the oleaginous marine microalga Nannochloropsis gaditana. Turbidostat cultures were used to selectively apply either N or P deprivation, controlling for variables including the light intensity. Global (cell-wide) changes in the proteome were measured using Tandem Mass Tag (TMT) and LC-MS/MS, whilst gene transcript expression of the same samples was quantified by Illumina RNA-sequencing. We detected 3423 proteins, where 1543 and 113 proteins showed significant changes in abundance in N and P treatments, respectively. The analysis includes the global correlation between proteomic and transcriptomic data, the regulation of subcellular proteomes in different compartments, gene/protein functional groups, and metabolic pathways. The results show that triacylglycerol (TAG) accumulation under nitrogen deprivation was associated with substantial downregulation of protein synthesis and photosynthetic activity. Oil accumulation was also accompanied by a diverse set of responses including the upregulation of diacylglycerol acyltransferase (DGAT), lipase, and lipid body associated proteins. Deprivation of phosphorus had comparatively fewer, weaker effects, some of which were linked to the remodeling of respiratory metabolism.
- Subjects :
- Proteomics
0106 biological sciences
0301 basic medicine
Bio Process Engineering
Tandem mass tag
Matematikk og Naturvitenskap: 400::Basale biofag: 470 [VDP]
01 natural sciences
nitrogen
Transcriptome
lcsh:Chemistry
transcriptomics
bioreactor
Nannochloropsis
phosphorus
lcsh:QH301-705.5
Spectroscopy
biology
Chemistry
Phosphorus
General Medicine
Computer Science Applications
Biochemistry
Proteome
Stramenopiles
Nitrogen
Bioreactor
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
proteomics
Downregulation and upregulation
Physical and Theoretical Chemistry
Transcriptomics
Molecular Biology
VLAG
Organic Chemistry
EPA
Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 [VDP]
Lipid Metabolism
biology.organism_classification
Metabolic pathway
Light intensity
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
TAG
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a8346b7f5b7af261169d59824a64db63
- Full Text :
- https://doi.org/10.3390/ijms21186946