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Integrin-mediated stimulation of monocyte chemotactic protein-1 expression
- Source :
- FEBS Letters. 414:221-225
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- We investigated whether activation of integrin receptors could modulate the expression of monocyte chemotactic protein-1 (MCP-1) in human hepatic stellate cells (HSC), mesenchymal cells responsible for extracellular matrix synthesis within the liver. When compared to non-adherent cells, HSC plated on collagen types I or IV, or fibronectin, showed increased MCP-1 gene expression and protein secretion in the conditioned medium. Increased MCP-1 secretion was also observed when cells were plated on dishes coated with a monoclonal antibody directed against the β1-integrin subunit, demonstrating that ligation of β1-integrins is sufficient to stimulate MCP-1 expression. Conversely, integrin-independent cell adhesion on poly-l-lysine did not modify MCP-1 secretion. Disruption of the actin cytoskeleton by cytochalasin D blocked the collagen-dependent increase in MCP-1 secretion. Chemotactic assay of HSC-conditioned medium showed that HSC plated on collagen secrete higher amounts of chemotactic factors for lymphomonocytes, and that MCP-1 accounts for the great majority of this effect. These findings indicate a novel mechanism of MCP-1 regulation possibly relevant in those conditions where HSC interact with an altered extracellular matrix.
- Subjects :
- Integrins
Integrin
Biophysics
In Vitro Techniques
Biology
Biochemistry
Mesoderm
Extracellular matrix
Monocyte chemotactic protein-1
Structural Biology
Cell Adhesion
Genetics
medicine
Humans
Secretion
Cell adhesion
Molecular Biology
Cells, Cultured
Chemokine CCL2
Hepatic stellate cell
Monocyte
Reproducibility of Results
Cell Biology
Actin cytoskeleton
Molecular biology
Extracellular Matrix
Fibronectins
Cell biology
Fibronectin
Chemotaxis, Leukocyte
medicine.anatomical_structure
Gene Expression Regulation
Liver
Chemokine
Leukocytes, Mononuclear
biology.protein
Collagen
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 414
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....a831512ad8b819780773b6ce9a94ce32