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Cognitive Function Improvement in Mouse Model of Alzheimer’s Disease Following Transcranial Direct Current Stimulation
- Source :
- Brain Sciences, Vol 10, Iss 547, p 547 (2020), Brain Sciences
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Anodal transcranial direct current stimulation (tDCS) is a painless noninvasive method that reportedly improves cognitive function in Alzheimer’s disease (AD) by stimulating the brain. However, its underlying mechanism remains unclear. Thus, the present study investigates the cognitive effects in a 5xFAD AD mouse model using electrophysiological and pathological methods. We used male 5xFAD C57BL/6J and male C57BL/6J wild-type mice; the dementia model was confirmed through DNA sequencing. The verified AD and wild-type mice were randomly assigned into four groups of five mice each: an induced AD group receiving tDCS treatment (Stim-AD), an induced AD group not receiving tDCS (noStim-AD), a non-induction group receiving tDCS (Stim-WT), and a non-induction group not receiving tDCS (noStim-WT). In the Stim group, mice received tDCS in the frontal bregma areas at an intensity of 200 µA for 20 min. After 2 weeks of treatment, we decapitated the mice, removed the hippocampus from the brain, confirmed its neuronal activation through excitatory postsynaptic potential (EPSP) recording, and performed molecular experiments on the remaining tissue using western blots. EPSP significantly increased in the Stim-AD group compared to that in the noStim-AD, which was comparable to that in the non-induced groups, Stim-WT and noStim-WT. There were no significant differences in cyclic amp-response element binding protein (CREB), phosphorylated CREB (pCREB), and Brain-derived neurotrophic factor (BDNF) levels in the Stim-AD group compared to those in the noStim-AD group. This study demonstrated that a tDCS in both frontal lobes of a transgenic 5xFAD mouse model affects long-term potentiation, indicating possible enhancement of cognitive function.
- Subjects :
- medicine.medical_specialty
medicine.medical_treatment
mouse model
Hippocampus
CREB
lcsh:RC321-571
Neurotrophic factors
Internal medicine
medicine
Dementia
direct current stimulation
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Transcranial direct-current stimulation
biology
business.industry
General Neuroscience
Communication
Long-term potentiation
medicine.disease
Electrophysiology
Endocrinology
Excitatory postsynaptic potential
biology.protein
business
cognitive enhancement
Alzheimer’s disease
Subjects
Details
- Language :
- English
- ISSN :
- 20763425
- Volume :
- 10
- Issue :
- 547
- Database :
- OpenAIRE
- Journal :
- Brain Sciences
- Accession number :
- edsair.doi.dedup.....a821040f810ba72cc1372c3e23f84a54