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Testing for ROS1 in non-small cell lung cancer: a review with recommendations
- Source :
- Bubendorf, L, Buettner, R, Al-Dayel, F, Dietel, M, Elmberger, G, Kerr, K, Lopez-Rios, F, Marchetti, A, Oz, B, Pauwels, P, Penault-Llorca, F, Rossi, G, Ryska, A & Thunnissen, E 2016, ' Testing for ROS1 in non-small cell lung cancer: a review with recommendations ', Virchows Archiv, vol. 469, no. 5, pp. 489-503 . https://doi.org/10.1007/s00428-016-2000-3, Virchows Archiv, Virchows Archiv, 469(5), 489-503. Springer Verlag
- Publication Year :
- 2016
-
Abstract
- Rearrangements of the ROS1 gene occur in 1-2 % of non-small cell lung cancers (NSCLCs). Crizotinib, a highly effective inhibitor of ROS1 kinase activity, is now FDA-approved for the treatment of patients with advanced ROS1-positive NSCLC. Consequently, focus on ROS1 testing is growing. Most laboratories currently rely on fluorescence in situ hybridisation (FISH) assays using a dual-colour break-apart probe to detect ROS1 rearrangements. Given the rarity of these rearrangements in NSCLC, detection of elevated ROS1 protein levels by immunohistochemistry may provide cost-effective screening prior to confirmatory FISH testing. Non-in situ testing approaches also hold potential as stand-alone methods or complementary tests, including multiplex real-time PCR assays and next-generation sequencing (NGS) platforms which include commercial test kits covering a range of fusion genes. In order to ensure high-quality biomarker testing, appropriate tissue handling, adequate control materials and participation in external quality assessment programmes are essential, irrespective of the testing technique employed. ROS1 testing is often only considered after negative tests for EGFR mutation and ALK gene rearrangement, based on the assumption that these oncogenic driver events tend to be exclusive. However, as the use of ROS1 inhibitors becomes routine, accurate and timely detection of ROS1 gene rearrangements will be critical for the optimal treatment of patients with NSCLC. As NGS techniques are introduced into routine diagnostic practice, ROS1 fusion gene testing will be provided as part of the initial testing package.
- Subjects :
- 0301 basic medicine
Lung Neoplasms
Predictive marker
RT-PCR
Computational biology
Biology
Pathology and Forensic Medicine
Fusion gene
03 medical and health sciences
0302 clinical medicine
Non-small cell lung cancer
Carcinoma, Non-Small-Cell Lung
Proto-Oncogene Proteins
ROS1
medicine
Humans
Multiplex
Kinase activity
Molecular Biology
Gene Rearrangement
Crizotinib
ALK Gene Rearrangement
Receptor Protein-Tyrosine Kinases
Oncogenes
Cell Biology
General Medicine
Protein-Tyrosine Kinases
Immunohistochemistry
Review and Perspectives
030104 developmental biology
030220 oncology & carcinogenesis
Cancer research
Biomarker (medicine)
Human medicine
Fluorescence in situ hybridisation
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 09456317
- Database :
- OpenAIRE
- Journal :
- Bubendorf, L, Buettner, R, Al-Dayel, F, Dietel, M, Elmberger, G, Kerr, K, Lopez-Rios, F, Marchetti, A, Oz, B, Pauwels, P, Penault-Llorca, F, Rossi, G, Ryska, A & Thunnissen, E 2016, ' Testing for ROS1 in non-small cell lung cancer: a review with recommendations ', Virchows Archiv, vol. 469, no. 5, pp. 489-503 . https://doi.org/10.1007/s00428-016-2000-3, Virchows Archiv, Virchows Archiv, 469(5), 489-503. Springer Verlag
- Accession number :
- edsair.doi.dedup.....a8200b5c5f8a6551aed333ca99265864