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Elucidation of veA-dependent genes associated with aflatoxin and sclerotial production in Aspergillus flavus by functional genomics
- Source :
- Applied microbiology and biotechnology. 76(5)
- Publication Year :
- 2007
-
Abstract
- The aflatoxin-producing fungi, Aspergillus flavus and A. parasiticus, form structures called sclerotia that allow for survival under adverse conditions. Deletion of the veA gene in A. flavus and A. parasiticus blocks production of aflatoxin as well as sclerotial formation. We used microarray technology to identify genes differentially expressed in wild-type veA and veA mutant strains that could be involved in aflatoxin production and sclerotial development in A. flavus. The DNA microarray analysis revealed 684 genes whose expression changed significantly over time; 136 of these were differentially expressed between the two strains including 27 genes that demonstrated a significant difference in expression both between strains and over time. A group of 115 genes showed greater expression in the wild-type than in the veA mutant strain. We identified a subgroup of veA-dependent genes that exhibited time-dependent expression profiles similar to those of known aflatoxin biosynthetic genes or that were candidates for involvement in sclerotial production in the wild type.
- Subjects :
- Aflatoxin
biology
Gene Expression Profiling
Wild type
food and beverages
Aspergillus flavus
Anthraquinones
General Medicine
Fungi imperfecti
Genomics
biology.organism_classification
Applied Microbiology and Biotechnology
Microbiology
Gene expression profiling
Fungal Proteins
Aflatoxins
Gene Expression Regulation, Fungal
Gene chip analysis
heterocyclic compounds
Functional genomics
Gene
Biotechnology
Gene Library
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 01757598
- Volume :
- 76
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Applied microbiology and biotechnology
- Accession number :
- edsair.doi.dedup.....a817f5fd29314275df8f6853819a9139