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A horizontal slice preparation for examining the functional connectivity of dorsal column fibres in mouse spinal cord
- Source :
- Journal of Neuroscience Methods. 200:113-120
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- In spinal cord injury (SCI) research, axon regeneration across spinal lesions is most often assessed using anatomical methods. It would be extremely advantageous, however, to examine the functional synaptic connectivity of regenerating fibres, using high-resolution electrophysiological methods. We have therefore developed a mouse horizontal spinal cord slice preparation that permits detailed analysis of evoked dorsal column (DCol) synaptic inputs on spinal neurons, using whole-cell patch clamp electrophysiology. This preparation allows us to characterise postsynaptic currents and potentials in response to electrical stimulation of DCol fibres, along with the intrinsic properties of spinal neurons. In addition, we demonstrate that low magnification calcium imaging can be used effectively to survey the spread of excitation from DCol stimulation in horizontal slices. This preparation is a potentially valuable tool for SCI research where confirmation of regenerated, functional synapses across a spinal lesion is critical.
- Subjects :
- Male
Patch-Clamp Techniques
Biophysics
Neural Conduction
In Vitro Techniques
Neurotransmission
Biology
Bicuculline
Inhibitory postsynaptic potential
Synaptic Transmission
Mice
Nerve Fibers
Slice preparation
Reaction Time
medicine
Animals
GABA-A Receptor Antagonists
Neurons, Afferent
Axon
Spinal cord injury
6-Cyano-7-nitroquinoxaline-2,3-dione
General Neuroscience
Glycine Agents
Strychnine
Spinal cord
medicine.disease
Electric Stimulation
Electrophysiology
medicine.anatomical_structure
Spinal Cord
Substantia Gelatinosa
Corticospinal tract
Calcium
Female
Excitatory Amino Acid Antagonists
Neuroscience
Subjects
Details
- ISSN :
- 01650270
- Volume :
- 200
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Methods
- Accession number :
- edsair.doi.dedup.....a814025a2f2420f76052e7091c2df2ec