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Construction and Nonclinical Testing of a Puumala Virus Synthetic M Gene-Based DNA Vaccine
- Source :
- Clinical and Vaccine Immunology. 20:218-226
- Publication Year :
- 2013
- Publisher :
- American Society for Microbiology, 2013.
-
Abstract
- Puumala virus (PUUV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). Although PUUV-associated HFRS does not result in high case-fatality rates, the social and economic impact is considerable. There is no licensed vaccine or specific therapeutic to prevent or treat HFRS. Here we report the synthesis of a codon-optimized, full-length M segment open reading frame and its cloning into a DNA vaccine vector to produce the plasmid pWRG/PUU-M(s2). pWRG/PUU-M(s2) delivered by gene gun produced high-titer neutralizing antibodies in hamsters and nonhuman primates. Vaccination with pWRG/PUU-M(s2) protected hamsters against infection with PUUV but not against infection by related HFRS-associated hantaviruses. Unexpectedly, vaccination protected hamsters in a lethal disease model of Andes virus (ANDV) in the absence of ANDV cross-neutralizing antibodies. This is the first evidence that an experimental DNA vaccine for HFRS can provide protection in a hantavirus lethal disease model.
- Subjects :
- Microbiology (medical)
Orthohantavirus
Hantavirus Infections
Clinical Biochemistry
Immunology
Andes virus
Viral Plaque Assay
Cross Reactions
Antibodies, Viral
Puumala virus
Cell Line
DNA vaccination
Viral Matrix Proteins
Neutralization Tests
Cricetinae
Chlorocebus aethiops
Vaccines, DNA
Animals
Immunology and Allergy
Vector (molecular biology)
Vero Cells
Hantavirus
Vaccines
biology
Viral Vaccine
Vaccination
virus diseases
Viral Vaccines
biology.organism_classification
Antibodies, Neutralizing
Macaca mulatta
Virology
Hemorrhagic Fever with Renal Syndrome
COS Cells
DNA, Viral
Hantavirus Infection
Subjects
Details
- ISSN :
- 1556679X and 15566811
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Clinical and Vaccine Immunology
- Accession number :
- edsair.doi.dedup.....a81303e1ac244be06b4d1b0b8375938d