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Emerging heterogeneous compartments by viruses in single bacterial cells
- Source :
- Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020), Nature Communications
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Spatial organization of biological processes allows for variability in molecular outcomes and coordinated development. Here, we investigate how organization underpins phage lambda development and decision-making by characterizing viral components and processes in subcellular space. We use live-cell and in situ fluorescence imaging at the single-molecule level to examine lambda DNA replication, transcription, virion assembly, and resource recruitment in single-cell infections, uniting key processes of the infection cycle into a coherent model of phage development encompassing space and time. We find that different viral DNAs establish separate subcellular compartments within cells, which sustains heterogeneous viral development in single cells. These individual phage compartments are physically separated by the E. coli nucleoid. Our results provide mechanistic details describing how separate viruses develop heterogeneously to resemble single-cell phenotypes.<br />Here, the authors apply live-cell and in situ fluorescence imaging at the single-molecule level to examine lambda DNA replication in single cells, finding that individual phage DNAs sequester host factors to their own vicinity and confine their replicated DNAs into separate compartments, suggesting that phage decision-making transcripts are spatially organized in separate compartments to allow distinct subcellular decisions to develop.
- Subjects :
- DNA Replication
0301 basic medicine
Transcription, Genetic
Science
viruses
General Physics and Astronomy
Computational biology
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Transcription (biology)
Escherichia coli
Nucleoid
Bacteriophages
lcsh:Science
Lysogeny
Multidisciplinary
biology
Virus Assembly
DNA replication
General Chemistry
Single-cell imaging
Lambda phage
Cellular noise
biology.organism_classification
Bacteriophage lambda
Phenotype
030104 developmental biology
chemistry
Virion assembly
DNA, Viral
lcsh:Q
030217 neurology & neurosurgery
DNA
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....a7d36efc2b7cbfa9b8d2c7b759e7de47