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Activated fibrinolytic enzymes in the synovial fluid during acute arthritis induced by urate crystal injection in dogs
- Source :
- Blood Coagulation & Fibrinolysis. 2:609-616
- Publication Year :
- 1991
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1991.
-
Abstract
- To identify the relationship of the severity of inflammation and fibrinolytic activity in arthritis, the fibrinolytic activity of synovial fluid was studied in acute experimental arthritis induced by injecting monosodium urate crystals into dogs' knee joints. The maximum activity in the synovial fluid was observed 6 h after crystal injection. It was inferred that the fibrinolytic activity was mainly due to plasminogen activator based on fibrin plate assays, substrate specificity, inhibitor effects and zymography. On the other hand, the activity of lysosomal enzymes (beta-glucuronidase and cathepsin G) reached a peak in the synovia after 12 h. Histological examination of the synovial membrane after 12 h also showed greater inflammation than at 6 h. The peak in fibrinolytic activity preceded the peak of lysosomal enzymes and histological changes. These results suggest that an increase in fibrinolytic activity by plasminogen activator may contribute to the development of an acute inflammatory response.
- Subjects :
- medicine.medical_specialty
Knee Joint
Molecular Sequence Data
Arthritis
Inflammation
Cathepsin G
Injections
chemistry.chemical_compound
Dogs
Internal medicine
Synovial Fluid
medicine
Animals
Synovial fluid
Zymography
Amino Acid Sequence
chemistry.chemical_classification
Fibrinolysis
Hematology
General Medicine
medicine.disease
Amides
Uric Acid
Enzyme Activation
medicine.anatomical_structure
Endocrinology
Enzyme
chemistry
Acute Disease
Chromatography, Gel
medicine.symptom
Synovial membrane
Crystallization
Lysosomes
Plasminogen activator
Subjects
Details
- ISSN :
- 09575235
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Blood Coagulation & Fibrinolysis
- Accession number :
- edsair.doi.dedup.....a7c1ea6b3e351d834d7abb6ab7fdbd40