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Effect of supersaturated oxygen emulsion treatment on chloropicrin-induced chemical injury in ex vivo rabbit cornea
- Source :
- Toxicology letters. 349
- Publication Year :
- 2021
-
Abstract
- With a possibility for the use of chemical weapons in battlefield or in terrorist activities, effective therapies against the devastating ocular injuries, from their exposure, are needed. Oxygen plays a vital role in ocular tissue preservation and wound repair. We tested the efficacy of supersaturated oxygen emulsion (SSOE) in reducing ex vivo corneal and keratocyte injury from chloropicrin (CP). CP, currently used as a pesticide, is a chemical threat agent like the vesicating mustard agents and causes severe corneal injury. Since our previous study in human corneal epithelial cells showed the treatment potential of SSOE (55 %), we further tested its efficacy in an ex vivo CP-induced rabbit corneal injury model. Corneas were exposed to CP (700 nmol) for 2 h, washed and cultured with or without SSOE for 24 h or 96 h. At 96 h post CP exposure, SSOE treatment presented a healing tendency of the corneal epithelial layer, and abrogated the CP-induced epithelial apoptotic cell death. SSOE treatment also reduced the CP induced DNA damage (H2A.X phosphorylation) and inflammatory markers (e.g. MMP9, IL-21, MIP-1β, TNFα). Further examination of the treatment efficacy of SSOE alone or in combination with other therapies in in vivo cornea injury models for CP and vesicants, is warranted.
- Subjects :
- 0301 basic medicine
Male
DNA damage
Apoptosis
MMP9
Pharmacology
Toxicology
Cornea
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Organ Culture Techniques
In vivo
Burns, Chemical
Hydrocarbons, Chlorinated
Medicine
Animals
Wound Healing
business.industry
Sulfur mustard
General Medicine
eye diseases
Oxygen
Eye Burns
030104 developmental biology
medicine.anatomical_structure
chemistry
Cytokines
Tumor necrosis factor alpha
Emulsions
sense organs
Rabbits
Inflammation Mediators
business
030217 neurology & neurosurgery
Ex vivo
Chemical Injury
DNA Damage
Subjects
Details
- ISSN :
- 18793169
- Volume :
- 349
- Database :
- OpenAIRE
- Journal :
- Toxicology letters
- Accession number :
- edsair.doi.dedup.....a7a6a0c25e2f8ed89a74f597a5ddfcf3