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Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica
- Source :
- Molecular Plant-Microbe Interactions®. 27:56-65
- Publication Year :
- 2014
- Publisher :
- Scientific Societies, 2014.
-
Abstract
- A small heat-shock protein gene, CpHsp24, of Cryphonectria parasitica was selected based on its expression pattern, which showed that it was tannic acid inducible and that its induction was severely hampered by a hypovirus. The predicted protein sequence of CpHsp24 consisted of a hallmark α-crystalline domain flanked by a variable N-terminal and a short C-terminal region. Disruption of CpHsp24 resulted in a slow growth rate under standard growth conditions. The CpHsp24-null mutant showed enhanced sensitivity to heat shock, which was consistent with Northern and Western analyses displaying the heat-shock induction of the CpHsp24 gene and protein, respectively. Virulence tests on the excised bark revealed a severe decrease in the necrotic area of the CpHsp24-null mutant. When the hypovirus was transferred, virus-containing CpHsp24-null progeny displayed severely retarded growth patterns with hypovirulent characteristics of reduced pigmentation and sporulation. Because the tannic-acid-inducible and hypoviral-suppressible expression and the severely impaired virulence are also characteristics of the laccase3 gene (lac3), lac3 expression in the CpHsp24-null mutant was also examined. The resulting lac3 induction was severely affected in the CpHsp24-null mutant, suggesting that CpHsp24 is important for lac3 induction and that CpHsp24 may act as a molecular chaperone for the lac3 protein.
- Subjects :
- Hot Temperature
Physiology
Mutant
Gene Expression
Virulence
macromolecular substances
Trees
Fungal Proteins
Ascomycota
Stress, Physiological
Gene Expression Regulation, Fungal
Chestnut blight
Heat shock protein
Gene expression
Botany
RNA Viruses
Cryphonectria
Hypovirus
Plant Diseases
Sequence Deletion
Regulation of gene expression
biology
Genetic Complementation Test
Laccase
General Medicine
biology.organism_classification
Molecular biology
Heat-Shock Proteins, Small
Protein Structure, Tertiary
Phenotype
Plant Bark
Cyperaceae
Tannins
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 19437706 and 08940282
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Molecular Plant-Microbe Interactions®
- Accession number :
- edsair.doi.dedup.....a78681c75daef442fbdb0037509df266