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FABP7 Facilitates Uptake of Docosahexaenoic Acid in Glioblastoma Neural Stem-like Cells
- Source :
- Nutrients, Nutrients, Vol 13, Iss 2664, p 2664 (2021), Volume 13, Issue 8
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Glioblastoma (GBM) is an aggressive tumor with a dismal prognosis. Neural stem-like cells contribute to GBM’s poor prognosis by driving drug resistance and maintaining cellular heterogeneity. GBM neural stem-like cells express high levels of brain fatty acid-binding protein (FABP7), which binds to polyunsaturated fatty acids (PUFAs) ω-6 arachidonic acid (AA) and ω-3 docosahexaenoic acid (DHA). Similar to brain, GBM tissue is enriched in AA and DHA. However, DHA levels are considerably lower in GBM tissue compared to adult brain. Therefore, it is possible that increasing DHA content in GBM, particularly in neural stem-like cells, might have therapeutic value. Here, we examine the fatty acid composition of patient-derived GBM neural stem-like cells grown as neurosphere cultures. We also investigate the effect of AA and DHA treatment on the fatty acid profiles of GBM neural stem-like cells with or without FABP7 knockdown. We show that DHA treatment increases DHA levels and the DHA:AA ratio in GBM neural stem-like cells, with FABP7 facilitating the DHA uptake. We also found that an increased uptake of DHA inhibits the migration of GBM neural stem-like cells. Our results suggest that increasing DHA content in the GBM microenvironment may reduce the migration/infiltration of FABP7-expressing neural stem-like cancer cells.
- Subjects :
- 0301 basic medicine
FABP7
neural stem-like cells
urologic and male genital diseases
chemistry.chemical_compound
0302 clinical medicine
Cell Movement
Tumor Microenvironment
TX341-641
Phospholipids
chemistry.chemical_classification
Gene knockdown
Nutrition and Dietetics
Arachidonic Acid
Brain Neoplasms
food and beverages
Brain
docosahexaenoic acid
Prognosis
3. Good health
B-FABP
Gene Expression Regulation, Neoplastic
Docosahexaenoic acid
030220 oncology & carcinogenesis
Fatty Acids, Unsaturated
Arachidonic acid
lipids (amino acids, peptides, and proteins)
Fatty Acid-Binding Protein 7
Polyunsaturated fatty acid
Docosahexaenoic Acids
Fatty Acid-Binding Proteins
fatty acids
Article
03 medical and health sciences
Neurosphere
Cell Line, Tumor
Fatty Acids, Omega-6
Fatty Acids, Omega-3
neurospheres
Humans
Nutrition. Foods and food supply
urogenital system
Tumor Suppressor Proteins
glioblastoma
Fatty acid
Biological Transport
nervous system diseases
030104 developmental biology
chemistry
Cancer cell
Cancer research
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 13
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....a76e44c4ef83aa3784a2de89dbf79ac6