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Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 5550, p 5550 (2020), International Journal of Molecular Sciences, Volume 21, Issue 15
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Lipoteichoic acid (LTA) is a cell wall component of Gram-positive bacteria. Limited data suggest that LTA is beneficial for bone regeneration in vitro. Thus, we used a mouse model of femoral defects to explore the effects of LTA on bone healing in vivo. Micro-computed tomography analysis and double-fluorochrome labeling were utilized to examine whether LTA can accelerate dynamic bone formation in vivo. The effects of LTA on osteoblastogenesis and osteoclastogenesis were also studied in vitro. LTA treatment induced prompt bone bridge formation, rapid endochondral ossification, and accelerated healing of fractures in mice with femoral bone defects. In vitro, LTA directly enhanced indicators of osteogenic factor-induced MC3T3-E1 cell differentiation, including alkaline phosphatase activity, calcium deposition and osteopontin expression. LTA also inhibited osteoclast activation induced by receptor activator of nuclear factor-kappa B ligand. We identified six molecules that may be associated with LTA-accelerated bone healing: monocyte chemoattractant protein 1, chemokine (C-X-C motif) ligand 1, cystatin C, growth/differentiation factor 15, endostatin and neutrophil gelatinase-associated lipocalin. Finally, double-fluorochrome, dynamic-labeling data indicated that LTA significantly enhanced bone-formation rates in vivo. In conclusion, our findings suggest that LTA has promising bone-regeneration properties.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Bone Regeneration
osteopontin
Osteoclasts
fluorochrome dynamic labeling
lcsh:Chemistry
Mice
0302 clinical medicine
Osteogenesis
Osteopontin
Femur
lcsh:QH301-705.5
Spectroscopy
030222 orthopedics
biology
Chemistry
Osteoblast
Cell Differentiation
General Medicine
respiratory system
Computer Science Applications
Cell biology
lipoteichoic acid
medicine.anatomical_structure
endochondral ossification
osteoclast
osteoblast
lipids (amino acids, peptides, and proteins)
Lipoteichoic acid
bone healing
alkaline phosphatase
femoral defect
Bone healing
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Osteoclast
In vivo
medicine
Animals
Humans
Physical and Theoretical Chemistry
Bone Resorption
Bone regeneration
Molecular Biology
Endochondral ossification
Osteoblasts
Organic Chemistry
RANK Ligand
X-Ray Microtomography
Teichoic Acids
carbohydrates (lipids)
stomatognathic diseases
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
fracture
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 21
- Issue :
- 5550
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a769add3e6011422ecffdf96b4cb30fc