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Kinetics of the cell biological changes occurring in the progression of DNA damage-induced senescence
- Source :
- Molecules and Cells. 31:539-546
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- Cellular senescence is characterized by cell-cycle arrest accompanied by various cell biological changes. Although these changes have been heavily relied on as senescence markers in numerous studies on senescence and its intervention, their underlying mechanisms and relationship to each other are poorly understood. Furthermore, the depth and the reversibility of those changes have not been addressed previously. Using flow cytometry coupled with confocal microscopy and Western blotting, we quantified various senescence-associated cellular changes and determined their time course profiles in MCF-7 cells undergoing DNA damage-induced senescence. The examined properties changed with several different kinetics patterns. Autofluorescence, side scattering, and the mitochondria content increased progressively and linearly. Cell volume, lysosome content, and reactive oxygen species (ROS) level increased abruptly at an early stage. Meanwhile, senescence associated β-galactosidase activity increased after a lag of a few days. In addition, during the senescence progression, lysosomes exhibited a loss of integrity, which may have been associated with the accumulation of ROS. The finding that various senescence phenotypes matured at different rates with different lag times suggests multiple independent mechanisms controlling the expression of senescence phenotypes. This type of kinetics study would promote the understanding of how cells become fully senescent and facilitate the screening of methods that intervene in cellular senescence.
- Subjects :
- Senescence
DNA damage
Cell
Mitochondrion
Biology
Ammonium Chloride
Permeability
Lipofuscin
Superoxides
Cell Line, Tumor
Lysosome
medicine
Humans
Molecular Biology
Cellular Senescence
Cell Size
Membrane Potential, Mitochondrial
Cell Cycle
Intracellular Membranes
Articles
Cell Biology
General Medicine
Cell cycle
beta-Galactosidase
Mitochondria
Cell biology
Kinetics
medicine.anatomical_structure
Doxorubicin
Cell culture
Lysosomes
Cell aging
DNA Damage
Subjects
Details
- ISSN :
- 02191032 and 10168478
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Molecules and Cells
- Accession number :
- edsair.doi.dedup.....a7637c70a7dfd9c9f582f5827fc95052