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Physiological and Proteomic Dissection of the Responses of Two Contrasting Wheat Genotypes to Nitrogen Deficiency
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 6, p 2119 (2020), International Journal of Molecular Sciences, Volume 21, Issue 6
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Nitrogen deficiency usually occurs along with aluminum toxicity in acidic soil, which is one of the major constraints for wheat production worldwide. In order to compare adaptive processes to N deficiency with different Al-tolerant wheat cultivars, we chose Atlas 66 and Scout 66 to comprehensively analyze the physiological responses to N deficiency, coupled with label-free mass spectrometry-based proteomics analysis. Results showed that both cultivars were comparable in most physiological indexes under N deficient conditions. However, the chlorophyll content in Scout 66 was higher than that of Atlas 66 under N deficiency. Further proteomic analysis identified 5592 and 5496 proteins in the leaves of Atlas 66 and Scout 66, respectively, of which 658 and 734 proteins were shown to significantly change in abundance upon N deficiency, respectively. The majority of the differentially expressed proteins were involved in cellular N compound metabolic process, photosynthesis, etc. Moreover, tetrapyrrole synthesis and sulfate assimilation were particularly enriched in Scout 66. Our findings provide evidence towards a better understanding of genotype-dependent responses under N deficiency which could help us to develop N efficient cultivars to various soil types.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chlorophyll
Proteomics
Genotype
Nitrogen
proteome
Biology
Photosynthesis
01 natural sciences
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
Species Specificity
Gene Expression Regulation, Plant
Soil pH
wheat
nitrogen starvation
Cultivar
Physical and Theoretical Chemistry
Sulfate assimilation
genes
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Triticum
Plant Proteins
Nitrogen deficiency
Organic Chemistry
Soil classification
General Medicine
Adaptation, Physiological
Computer Science Applications
Plant Leaves
030104 developmental biology
Biochemistry
lcsh:Biology (General)
lcsh:QD1-999
leaf chlorophyll
Proteome
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 21
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a75eb42b1338f027161c933857dff43f