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Nerolidol Mitigates Colonic Inflammation: An Experimental Study Using both In Vivo and In Vitro Models
- Source :
- Nutrients, Volume 12, Issue 7, Nutrients, Vol 12, Iss 2032, p 2032 (2020)
- Publication Year :
- 2020
-
Abstract
- Nerolidol (NED) is a naturally occurring sesquiterpene alcohol present in various plants with potent anti-inflammatory effects. In the current study, we investigated NED as a putative anti-inflammatory compound in an experimental model of colonic inflammation. C57BL/6J male black mice (C57BL/6J) were administered 3% dextran sodium sulfate (DSS) in drinking water for 7 days to induce colitis. Six groups received either vehicle alone or DSS alone or DSS with oral NED (50, 100, and 150 mg/kg body weight/day by oral gavage) or DSS with sulfasalazine. Disease activity index (DAI), colonic histology, and biochemical parameters were measured. TNF-&alpha<br />treated HT-29 cells were used as in vitro model of colonic inflammation to study NED (25 &micro<br />M and 50 &micro<br />M). NED significantly decreased the DAI and reduced the inflammation-associated changes in colon length as well as macroscopic and microscopic architecture of the colon. Changes in tissue Myeloperoxidase (MPO) concentrations, neutrophil and macrophage mRNA expression (CXCL2 and CCL2), and proinflammatory cytokine content (IL-1&beta<br />IL-6, and TNF-&alpha<br />) both at the protein and mRNA level were significantly reduced by NED. The increase in content of the proinflammatory enzymes, COX-2 and iNOS induced by DSS were also significantly inhibited by NED along with tissue nitrate levels. NED promoted Nrf2 nuclear translocation dose dependently. NED significantly increased antioxidant enzymes activity (Superoxide dismutase (SOD) and Catalase (CAT)), Hemeoxygenase-1 (HO-1), and SOD3 mRNA levels. NED treatment in TNF-&alpha<br />challenged HT-29 cells significantly decreased proinflammatory chemokines (CXCL1, IL-8, CCL2) and COX-2 mRNA levels. NED supplementation attenuates colon inflammation through its potent antioxidant and anti-inflammatory activity both in in vivo and in vitro models of colonic inflammation.
- Subjects :
- 0301 basic medicine
Male
nerolidol
Neutrophils
colitis
Phytochemicals
Anti-Inflammatory Agents
Administration, Oral
phytochemical
Pharmacology
Antioxidants
0302 clinical medicine
Nutrition and Dietetics
biology
Chemistry
Myeloperoxidase
Cytokines
medicine.symptom
Inflammation Mediators
lcsh:Nutrition. Foods and food supply
HT29 Cells
Sesquiterpenes
SOD3
Colon
NF-E2-Related Factor 2
lcsh:TX341-641
Inflammation
CCL2
Article
Proinflammatory cytokine
Superoxide dismutase
03 medical and health sciences
In vivo
inflammatory bowel disease
medicine
Animals
Humans
Colitis
Peroxidase
bioactive compounds
inflammatory molecular mechanisms
Macrophages
medicine.disease
Inflammatory Bowel Diseases
digestive system diseases
Mice, Inbred C57BL
stomatognathic diseases
Disease Models, Animal
030104 developmental biology
biology.protein
030217 neurology & neurosurgery
Food Science
Phytotherapy
Subjects
Details
- ISSN :
- 20726643
- Volume :
- 12
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....a75d5f9ddc6404908c92b111fa32e589