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Calcium signals in guard cells enhance the efficiency by which abscisic acid triggers stomatal closure
- Source :
- The New Phytologist
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Summary During drought, abscisic acid (ABA) induces closure of stomata via a signaling pathway that involves the calcium (Ca2+)‐independent protein kinase OST1, as well as Ca2+‐dependent protein kinases. However, the interconnection between OST1 and Ca2+ signaling in ABA‐induced stomatal closure has not been fully resolved.ABA‐induced Ca2+ signals were monitored in intact Arabidopsis leaves, which express the ratiometric Ca2+ reporter R‐GECO1‐mTurquoise and the Ca2+‐dependent activation of S‐type anion channels was recorded with intracellular double‐barreled microelectrodes.ABA triggered Ca2+ signals that occurred during the initiation period, as well as in the acceleration phase of stomatal closure. However, a subset of stomata closed in the absence of Ca2+ signals. On average, stomata closed faster if Ca2+ signals were elicited during the ABA response. Loss of OST1 prevented ABA‐induced stomatal closure and repressed Ca2+ signals, whereas elevation of the cytosolic Ca2+ concentration caused a rapid activation of SLAC1 and SLAH3 anion channels.Our data show that the majority of Ca2+ signals are evoked during the acceleration phase of stomatal closure, which is initiated by OST1. These Ca2+ signals are likely to activate Ca2+‐dependent protein kinases, which enhance the activity of S‐type anion channels and boost stomatal closure.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
stomata
Arabidopsis
chemistry.chemical_element
Plant Science
Calcium
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Cytosol
Ca2+‐indicator
cytosolic Ca2+ signals
Guard cell
Calcium Signaling
Protein kinase A
Abscisic acid
Full Paper
SLAC1 and SLAH3 anion channels
Arabidopsis Proteins
Kinase
Research
fungi
R‐GECO1‐mTurquoise
food and beverages
Full Papers
OST1 protein kinase
030104 developmental biology
chemistry
Plant Stomata
Biophysics
abscisic acid (ABA)
Calcium Channels
Signal transduction
Intracellular
Abscisic Acid
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14698137 and 0028646X
- Volume :
- 224
- Database :
- OpenAIRE
- Journal :
- New Phytologist
- Accession number :
- edsair.doi.dedup.....a74e16811c57dc126951e285899a0eb0