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Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo
- Source :
- Histochemistry and Cell Biology
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical technique that non-invasively resolves single molecules through recording the signal intensity at the femtolitre scale. However, recording the behaviour of these biomolecules using in vitro-based assays often fails to recapitulate the full range of variables in vivo that directly confer dynamics. Therefore, there has been an increasing interest in observing the state of these biomolecules within living organisms such as the zebrafish Danio rerio. In this review, we explore the advancements of FCS within the zebrafish and compare and contrast these findings to those found in vitro.
- Subjects :
- 0301 basic medicine
Histology
Danio
Fluorescence correlation spectroscopy
Review
Protein–protein interactions
Molecular Dynamics Simulation
Protein–protein interaction
03 medical and health sciences
0302 clinical medicine
In vivo
Animals
Molecular Biology
Zebrafish
chemistry.chemical_classification
Microscopy
Fluorescent correlation spectroscopy (FCS)
biology
Biomolecule
Proteins
Cell Biology
biology.organism_classification
In vitro
Medical Laboratory Technology
Spectrometry, Fluorescence
030104 developmental biology
chemistry
Biophysics
lipids (amino acids, peptides, and proteins)
030217 neurology & neurosurgery
Function (biology)
Subjects
Details
- ISSN :
- 1432119X and 09486143
- Volume :
- 154
- Database :
- OpenAIRE
- Journal :
- Histochemistry and Cell Biology
- Accession number :
- edsair.doi.dedup.....a740fc9152c922290a406b3d08a4edec