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Identification of the metabolites of isochlorogenic acid A in rats by UHPLC-Q-Exactive Orbitrap MS
- Source :
- Pharmaceutical Biology, article-version (VoR) Version of Record, Pharmaceutical Biology, Vol 58, Iss 1, Pp 992-998 (2020)
- Publication Year :
- 2020
-
Abstract
- Content Isochlorogenic acid A, one of the main components of Duhaldea nervosa (Wallich ex Candolle) A. Anderberg (Asteraceae), is a folk medicine used to treat a variety of diseases including fracture and rheumatoid arthritis. Despite its widespread use, the metabolism of isochlorogenic acid A in vivo has not been fully studied. Objective An analytical strategy based on UHPLC-Q-Exactive Orbitrap MS is proposed for the detection and identification of the metabolites of isochlorogenic acid A in rats. Materials and methods Six male Sprague-Dawley rats (180 ± 20 g) were randomly divided into two groups. Then, blood and tissue samples were obtained after oral administration of isochlorogenic acid A (200 mg/kg). All the samples were pre-treated by the Solid Phase Extraction (SPE) method. Next, the samples were analysed by UHPLC-Q-Exactive Orbitrap MS. Finally, the metabolites were identified based on the metabolomic workflow template. Results A total of 33 metabolites were identified in rat plasma, with 30 of them being reported for the first time. The distribution of all metabolites in tissues was first investigated, three of them were widely distributed in liver, lungs, and kidneys. The corresponding reactions including methylation, hydrolysis, sulphate conjugation, glucuronide conjugation, as well as their composite reactions, are reported in this study. Discussion and conclusions This method has wide-scale application prospects in the identification of metabolites. Considering that limited research has been conducted in this area, this study proposes metabolic pathways to further understand mechanisms of isochlorogenic acid A and the forms that are truly effective in vivo.
- Subjects :
- Male
Duhaldea
relative abundance
Pharmaceutical Science
Administration, Oral
RM1-950
Asteraceae
030226 pharmacology & pharmacy
01 natural sciences
Mass Spectrometry
Article
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Liquid chromatography–mass spectrometry
mental disorders
Drug Discovery
distribution
Animals
metabolic pathways
Tissue Distribution
35-DiCQA
Chromatography, High Pressure Liquid
Pharmacology
Folk medicine
Chromatography
biology
Chemistry
Orbitrap ms
Solid Phase Extraction
General Medicine
biology.organism_classification
0104 chemical sciences
Isochlorogenic acid
Rats
LC-MS
010404 medicinal & biomolecular chemistry
Complementary and alternative medicine
Q exactive
Molecular Medicine
β-glucuronidase
Therapeutics. Pharmacology
Chlorogenic Acid
Research Article
metabolomic
Subjects
Details
- ISSN :
- 17445116
- Volume :
- 58
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical biology
- Accession number :
- edsair.doi.dedup.....a73047f137310d0d914be1004d2d4cc2