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FOLLICLE-LIKE MODEL BY GRANULOSA CELL ENCAPSULATION IN A BARIUM ALGINATE/PROTAMINE MEMBRANE
FOLLICLE-LIKE MODEL BY GRANULOSA CELL ENCAPSULATION IN A BARIUM ALGINATE/PROTAMINE MEMBRANE
- Publication Year :
- 2005
-
Abstract
- Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.
- Subjects :
- medicine.medical_specialty
GRANULOSA CELLS
Time Factors
Alginates
Swine
Granulosa cell
Cell Culture Techniques
Biology
Models, Biological
3D cell culture
Follicle
Tissue engineering
Glucuronic Acid
Internal medicine
Monolayer
medicine
Animals
Protamines
Tissue Engineering
Hexuronic Acids
General Engineering
Membranes, Artificial
Protamine
BARIUM ALGINATE CAPSULES
Cell biology
In vitro maturation
Endocrinology
IVM
Cell culture
FOLLICLE
biology.protein
Microscopy, Electron, Scanning
Oocytes
Settore VET/02 - Fisiologia Veterinaria
Cattle
Female
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a6fbb5e4a00bcd13456986b2864f15a3