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FOLLICLE-LIKE MODEL BY GRANULOSA CELL ENCAPSULATION IN A BARIUM ALGINATE/PROTAMINE MEMBRANE

FOLLICLE-LIKE MODEL BY GRANULOSA CELL ENCAPSULATION IN A BARIUM ALGINATE/PROTAMINE MEMBRANE

Authors :
Giovanna Galeati
U. Conte
Simona Villani
Massimo Faustini
Eleonora Munari
Marcella Spinaci
Pier Attilio Accorsi
Maria Luisa Torre
Annalia Asti
Vincenzo Russo
Daniele Vigo
VIGO D.
VILLANI S.
FAUSTINI M.
ACCORSI P.A.
GALEATI G.
SPINACI M.
MUNARI E.
RUSSO V.
ASTI A.
CONTE U.
TORRE ML.
Publication Year :
2005

Abstract

Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....a6fbb5e4a00bcd13456986b2864f15a3