Back to Search
Start Over
Decreased Frequencies of Peripheral Blood CD4+CD25+CD127-Foxp3+ in Patients with Graves' Disease and Graves' Orbitopathy: Enhancing Effect of Insulin Growth Factor-1 on Treg Cells
- Source :
- Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 49(3)
- Publication Year :
- 2017
-
Abstract
- Graves' orbitopathy (GO) is characterized by orbital T cell infiltration. We evaluated the regulatory T (Treg) cell fractions induced with IGF-1 in Graves' disease (GD) with and without GO. Peripheral blood mononuclear cells (PBMCs) were obtained from 13 patients with GD without eye manifestations; 10 patients with active GO; and 12 patients with nodular goiter (NG). All the patients from GD, GO, and NG were subclinical hyperthyroid. We analyzed the expression of Treg cell markers (CD4, CD25, CD127-, Foxp3) on T cells and their ability to respond to IGF-1 stimulation. In patients with GD without GO, we found lowered percentages of CD4+ Foxp3+ cells, as compared to nodular goiter 1.77 vs. 5.42% (p=0.0276). Similarly, significantly reduced frequencies of CD4+CD25+CD127-Foxp3+ and CD4+CD25+CD127- cells were observed in GD patients as compared to nodular goiter patients with hyperthyreosis, (0.7 vs. 1.48%) (p=0.0071) and (14.5 vs. 37.2%) (p=0.0051), respectively. In GO with active GO, only the percentage of CD4+CD25+CD127- cells was found to be decreased versus nodular goiter (9.35 vs. 37.2) (p=0.0275). Stimulation of PBMC derived from GO patients with IGF-1 resulted in significant increase of frequency of both CD4+ Foxp3+ and CD4+CD25+CD127- Foxp3 cells. Decreased frequencies of peripheral blood CD4+CD25+CD127-Foxp3+ in patients with GD and GO could be an useful marker of autoimmune process and perhaps a possible target for future therapies. This is the first study demonstrating Treg-enhancing effects of IGF-1. Thus IGF-1 can be accounted for modulating Treg cell-related action in GO.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Goiter
Endocrinology, Diabetes and Metabolism
Graves' disease
Clinical Biochemistry
chemical and pharmacologic phenomena
Stimulation
medicine.disease_cause
Biochemistry
Peripheral blood mononuclear cell
T-Lymphocytes, Regulatory
Autoimmunity
Interleukin-7 Receptor alpha Subunit
03 medical and health sciences
Endocrinology
Internal medicine
medicine
Humans
IL-2 receptor
Insulin-Like Growth Factor I
Interleukin-7 receptor
Aged
business.industry
Biochemistry (medical)
Interleukin-2 Receptor alpha Subunit
FOXP3
hemic and immune systems
Forkhead Transcription Factors
General Medicine
Middle Aged
medicine.disease
Graves Ophthalmopathy
030104 developmental biology
CD4 Antigens
Female
business
Subjects
Details
- ISSN :
- 14394286
- Volume :
- 49
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
- Accession number :
- edsair.doi.dedup.....a6fb69e348fef53e87af06f70669bef7