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A strategy to replace the mouse bioassay for detecting and identifying lipophilic marine biotoxins by combining the neuro-2a bioassay and LC-MS/MS analysis
- Source :
- Marine Drugs, 16(12), Marine Drugs, Volume 16, Issue 12, Marine Drugs, Vol 16, Iss 12, p 501 (2018), Marine Drugs 16 (2018) 12
- Publication Year :
- 2018
-
Abstract
- Marine biotoxins in fish and shellfish can cause several symptoms in consumers, such as diarrhea, amnesia, or even death by paralysis. Monitoring programs are in place for testing shellfish on a regular basis. In some countries testing is performed using the so-called mouse bioassay, an assay that faces ethical concerns not only because of animal distress, but also because it lacks specificity and results in high amounts of false positives. In Europe, for lipophilic marine biotoxins (LMBs), a chemical analytical method using LC-MS/MS was developed as an alternative and is now the reference method. However, safety is often questioned when relying solely on such a method, and as a result, the mouse bioassay might still be used. In this study the use of a cell-based assay for screening, i.e., the neuro-2a assay, in combination with the official LC-MS/MS method was investigated as a new alternative strategy for the detection and quantification of LMBs. To this end, samples that had been tested previously with the mouse bioassay were analyzed in the neuro-2a bioassay and the LC-MS/MS method. The neuro-2a bioassay was able to detect all LMBs at the regulatory levels and all samples that tested positive in the mouse bioassay were also suspect in the neuro-2a bioassay. In most cases, these samples contained toxin levels (yessotoxins) that explain the outcome of the bioassay but did not exceed the established maximum permitted levels.
- Subjects :
- 0301 basic medicine
Novel Foods & Agrochains
Pharmaceutical Science
Novel Foods & Agroketens
01 natural sciences
Mice
BU Contaminants & Toxins
Mouse bioassay
Tandem Mass Spectrometry
Drug Discovery
False positive paradox
Bioassay
BU Toxicology, Novel Foods & Agrochains
lcsh:QH301-705.5
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Chromatography, High Pressure Liquid
Oxocins
BU Toxicology
BU Toxicologie, Novel Foods & Agroketens
Ethical concerns
Fish
Biological Assay
Alternative strategy
BU Toxicologie
BU Contaminanten & Toxines
Mollusk Venoms
Biology
Animal Testing Alternatives
Article
03 medical and health sciences
BU Authenticity & Bioassays
Cell Line, Tumor
Lc ms ms
Animals
Shellfish Poisoning
False Positive Reactions
Shellfish
VLAG
Chromatography
010401 analytical chemistry
Lipophilic marine toxins
0104 chemical sciences
Bivalvia
030104 developmental biology
BU Authenticiteit & Bioassays
lcsh:Biology (General)
Marine Toxins
Neuro-2a assay
Subjects
Details
- Language :
- English
- ISSN :
- 16603397
- Database :
- OpenAIRE
- Journal :
- Marine Drugs, 16(12), Marine Drugs, Volume 16, Issue 12, Marine Drugs, Vol 16, Iss 12, p 501 (2018), Marine Drugs 16 (2018) 12
- Accession number :
- edsair.doi.dedup.....a6de98ea6e676b14b0180e5ccbf9a458