Back to Search
Start Over
hCAS/CSE1L regulates RAD51 distribution and focus formation for homologous recombinational repair
- Source :
- Genes to cells : devoted to molecularcellular mechanisms. 20(9)
- Publication Year :
- 2015
-
Abstract
- Homologous recombinational repair (HR) is one of the major repair systems for DNA double-strand breaks. RAD51 is a key molecule in HR, and the RAD51 concentration in the cell nucleus increases after DNA damage induction. However, the mechanism that regulates the intracellular distribution of RAD51 is still unclear. Here, we show that hCAS/CSE1L associates with RAD51 in human cells. We found that hCAS/CSE1L negatively regulates the nuclear protein level of RAD51 under normal conditions. hCAS/CSE1L is also required to repress the DNA damage-induced focus formation of RAD51. Moreover, we show that hCAS/CSE1L plays roles in the regulation of the HR activity and in chromosome stability. These findings suggest that hCAS/CSE1L is responsible for controlling the HR activity by directly interacting with RAD51.
- Subjects :
- DNA damage
genetic processes
RAD51
Biology
chemistry.chemical_compound
Cellular Apoptosis Susceptibility Protein
Cell Line, Tumor
Genetics
medicine
Homologous chromosome
Humans
DNA Breaks, Double-Stranded
Nuclear protein
Homologous Recombination
Cell Nucleus
Chromosome Aberrations
Recombinational DNA Repair
Cell Biology
Molecular biology
enzymes and coenzymes (carbohydrates)
Cell nucleus
medicine.anatomical_structure
chemistry
health occupations
Rad51 Recombinase
biological phenomena, cell phenomena, and immunity
Homologous recombination
DNA
Intracellular
Subjects
Details
- ISSN :
- 13652443
- Volume :
- 20
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Genes to cells : devoted to molecularcellular mechanisms
- Accession number :
- edsair.doi.dedup.....a6b2f65fadae48d0ee7a017f4fe68e87