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Toward Automated Enzymatic Synthesis of Oligosaccharides
Toward Automated Enzymatic Synthesis of Oligosaccharides
- Source :
- Chemical Reviews. 118:8151-8187
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Oligosaccharides together with oligonucleotides and oligopeptides comprise the three major classes of natural biopolymers. Automated systems for oligonucleotide and oligopeptide synthesis have significantly advanced developments in biological science by allowing nonspecialists to rapidly and easily access these biopolymers. Researchers have endeavored for decades to develop a comparable general automated system to synthesize oligosaccharides. Such a system would have a revolutionary impact on the understanding of the roles of glycans in biological systems. The main challenge to achieving automated synthesis is the lack of general synthetic methods for routine synthesis of glycans. Currently, the two main methods to access homogeneous glycans and glycoconjugates are chemical synthesis and enzymatic synthesis. Enzymatic glycosylation can proceed stereo- and regiospecifically without protecting group manipulations. Moreover, the reaction conditions of enzyme-catalyzed glycosylations are extremely mild when compared to chemical glycosylations. Over the past few years methodology toward the automated chemical synthesis of oligosaccharides has been developed. Conversely, while automated enzymatic synthesis is conceptually possible, it is not as well developed. The goal of this survey is to provide a foundation on which continued technological advancements can be made to promote the automated enzymatic synthesis of oligosaccharides.
- Subjects :
- 0301 basic medicine
Glycan
Glycosylation
Glycoconjugate
Oligosaccharides
Chemistry Techniques, Synthetic
01 natural sciences
Chemical synthesis
Catalysis
Automation
03 medical and health sciences
chemistry.chemical_compound
Protecting group
chemistry.chemical_classification
biology
010405 organic chemistry
Oligonucleotide
Glycosyltransferases
Stereoisomerism
General Chemistry
Enzymatic synthesis
0104 chemical sciences
030104 developmental biology
Carbohydrate Sequence
Biochemistry
chemistry
Homogeneous
biology.protein
Glycoconjugates
Subjects
Details
- ISSN :
- 15206890 and 00092665
- Volume :
- 118
- Database :
- OpenAIRE
- Journal :
- Chemical Reviews
- Accession number :
- edsair.doi.dedup.....a6980e78b7b34e0b1b06b55e2721fd77
- Full Text :
- https://doi.org/10.1021/acs.chemrev.8b00066