Back to Search Start Over

Toward Automated Enzymatic Synthesis of Oligosaccharides

Toward Automated Enzymatic Synthesis of Oligosaccharides

Authors :
Garrett Edmunds
Jiabin Zhang
Hailiang Zhu
Yun Kong
Liuqing Wen
Xianwei Liu
Peng George Wang
Christopher Gibbons
Madhusudhan Reddy Gadi
Junqiang Fang
Source :
Chemical Reviews. 118:8151-8187
Publication Year :
2018
Publisher :
American Chemical Society (ACS), 2018.

Abstract

Oligosaccharides together with oligonucleotides and oligopeptides comprise the three major classes of natural biopolymers. Automated systems for oligonucleotide and oligopeptide synthesis have significantly advanced developments in biological science by allowing nonspecialists to rapidly and easily access these biopolymers. Researchers have endeavored for decades to develop a comparable general automated system to synthesize oligosaccharides. Such a system would have a revolutionary impact on the understanding of the roles of glycans in biological systems. The main challenge to achieving automated synthesis is the lack of general synthetic methods for routine synthesis of glycans. Currently, the two main methods to access homogeneous glycans and glycoconjugates are chemical synthesis and enzymatic synthesis. Enzymatic glycosylation can proceed stereo- and regiospecifically without protecting group manipulations. Moreover, the reaction conditions of enzyme-catalyzed glycosylations are extremely mild when compared to chemical glycosylations. Over the past few years methodology toward the automated chemical synthesis of oligosaccharides has been developed. Conversely, while automated enzymatic synthesis is conceptually possible, it is not as well developed. The goal of this survey is to provide a foundation on which continued technological advancements can be made to promote the automated enzymatic synthesis of oligosaccharides.

Details

ISSN :
15206890 and 00092665
Volume :
118
Database :
OpenAIRE
Journal :
Chemical Reviews
Accession number :
edsair.doi.dedup.....a6980e78b7b34e0b1b06b55e2721fd77
Full Text :
https://doi.org/10.1021/acs.chemrev.8b00066