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Hyaluronan suppresses mechanical stress-induced expression of catabolic enzymes by human chondrocytes via inhibition of IL-1β production and subsequent NF-κB activation
- Source :
- Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 64(3-4)
- Publication Year :
- 2014
-
Abstract
- To investigate the inhibitory effect of hyaluronan (HA) on mechanical stress- induced expression of a disintegrin and metalloproteinase with thrombospondin type 1 motifs (ADAMTS)-4, -5 and matrix metalloproteinase (MMP)-13 by human chondrocytes.Normal human articular chondrocytes were pre-incubated with or without 1.0 mg/mL HA (2700 kDa) for 12 h at 37 °C in stretch chambers, then they were exposed to uni-axial cyclic tensile strain (CTS, 0.5 Hz, 10% elongation). The expression of ADAMTS-4, -5, and MMP-13 were analyzed by real-time polymerase chain reaction and Immunocytochemistry. The concentration of IL-1β in the supernatant was measured using enzyme-linked immunosorbent assay (ELISA). The nuclear translocation of runt-related transcription factor 2 (RUNX-2) and nuclear factor-κB (NF-κB) was examined by ELISA and immunocytochemistry, and phosphorylation of NF-κB was examined by western blotting.HA inhibited mRNA expression of ADAMTS-4, -5, and MMP13 after 24 h CTS via inhibition of IL-1β secretion and NF-κB activation. However, HA failed to inhibit CTS-induced RUNX-2 expression and subsequent expression of ADAMTS-5 and MMP-13 1 h after CTS.Our results demonstrated that HA significantly suppressed mechanical stress-induced expression of catabolic proteases by inhibition of the NF-κB-IL-1β pathway, but did not suppress mechanical stress-induced RUNX-2 signaling.
- Subjects :
- Adult
Adolescent
Disintegrins
Immunology
Interleukin-1beta
Core Binding Factor Alpha 1 Subunit
Matrix metalloproteinase
In Vitro Techniques
Chondrocyte
Chondrocytes
Matrix Metalloproteinase 13
Disintegrin
medicine
Humans
RNA, Messenger
Hyaluronic Acid
Cells, Cultured
Aggrecanase
Pharmacology
Metalloproteinase
Thrombospondin
biology
Chemistry
ADAMTS
NF-kappa B
ADAMTS4 Protein
Cell biology
carbohydrates (lipids)
ADAM Proteins
medicine.anatomical_structure
biology.protein
ADAMTS5 Protein
Stress, Mechanical
Procollagen N-Endopeptidase
Signal Transduction
Subjects
Details
- ISSN :
- 1420908X
- Volume :
- 64
- Issue :
- 3-4
- Database :
- OpenAIRE
- Journal :
- Inflammation research : official journal of the European Histamine Research Society ... [et al.]
- Accession number :
- edsair.doi.dedup.....a696e72c50fe33bc3d11870663536ea7