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The APPEESFRS Peptide, Restricted by the HLA-B*35:01 Molecule, and the APPEESFRF Variant Derived from an Autologous HIV-1 Strain Induces Polyfunctional Responses in CD8+ T Cells
- Source :
- BioResearch Open Access, BioResearch Open Access, Vol 4, Iss 1, Pp 115-120 (2015)
- Publication Year :
- 2015
-
Abstract
- Numerous reports have focused on consensus peptides to determine CD8+ T-cell responses; however, few studies evaluated the functional profile using peptides derived from circulating strains of a specific region. We determined the effector profile and maturation phenotype of CD8+ T-cells targeting the consensus APPEESFRS (AS9) epitope and its variant APPEESFRF (AF9), previously identified. The free energy of binding, maturation phenotype, and polyfunctional profile of both peptides were similar. The magnitude of CD8+ T-cell responses to AF9 was greater than the one elicited by AS9, although the difference was not significant. The polyfunctional profile of AF9 was characterized by CD107a/interleukin-2 (IL-2)/macrophage inflammatory protein beta (MIP1β) and by interferon gamma (IFNγ)/MIP1β/tumor necrosis factor alpha (TNFα) in response to AS9. TNFα production was significantly higher in response to AF9 than to AS9, and there was a negative correlation between the absolute number of CD8+ T-cell-producing TNFα and the plasma human immunodeficiency virus (HIV) load, suggesting a role of this cytokine in the control of HIV replication.
- Subjects :
- chemistry.chemical_classification
functional profile
human immunodeficiency virus
medicine.medical_treatment
lcsh:R
lcsh:Medicine
Peptide
Biology
HIV-specific CD8+ T-cells
General Biochemistry, Genetics and Molecular Biology
Epitope
Cytokine
lcsh:Biology (General)
chemistry
Immunology
medicine
peptides
Cytotoxic T cell
Tumor necrosis factor alpha
Interferon gamma
lcsh:QH301-705.5
Macrophage inflammatory protein
CD8
Rapid Communication
medicine.drug
Subjects
Details
- ISSN :
- 21647844
- Volume :
- 4
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BioResearch open access
- Accession number :
- edsair.doi.dedup.....a6957b2e91477598ef87f0a8b555399f