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Mode of Transmission Affects the Sensitivity of Human Immunodeficiency Virus Type 1 to Restriction by Rhesus TRIM5α
- Source :
- Journal of Virology. 82:11117-11128
- Publication Year :
- 2008
- Publisher :
- American Society for Microbiology, 2008.
-
Abstract
- Rhesus TRIM5alpha (rhTRIM5alpha), but not human TRIM5alpha (huTRIM5alpha), potently inhibits human immunodeficiency virus (HIV) infection and is thus a potentially valuable therapeutic tool. Primary human CD4 T cells engineered to express rhTRIM5alpha were highly resistant to cell-free HIV type 1 (HIV-1) infection. However, when cocultured with unmodified T cells, rhTRIM5alpha-expressing cells became highly permissive to HIV-1 infection. Physical separation of rhTRIM5alpha-expressing cells and unmodified cells revealed that rhTRIM5alpha efficiently restricts cell-free but not cell-associated HIV transmission. Furthermore, we observed that HIV-infected human cells could infect rhesus CD4 T cells by cell-to-cell contact, but the infection was self-limiting. Subsequently, we noted that a spreading infection ensued when HIV-1-infected rhTRIM5alpha-expressing human cells were cultured with huTRIM5alpha- but not rhTRIM5alpha-expressing cells. Our results suggest that cell-associated HIV transmission in humans is blocked only when both donor and recipient cells express rhTRIM5alpha. These studies further define the role of rhTRIM5alpha in cell-free and cell-associated HIV transmission and delineate the utility of rhTRIM5alpha in anti-HIV therapy.
- Subjects :
- CD4-Positive T-Lymphocytes
Ubiquitin-Protein Ligases
Immunology
Microbiology
Virus
law.invention
Acquired immunodeficiency syndrome (AIDS)
law
Virology
Immunopathology
medicine
Animals
Humans
Permissive
Sida
Cells, Cultured
biology
Proteins
virus diseases
medicine.disease
biology.organism_classification
Macaca mulatta
Coculture Techniques
Immunity, Innate
Virus-Cell Interactions
Transmission (mechanics)
Insect Science
Lentivirus
HIV-1
Viral disease
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....a6902fa90d80f7f195428b0eea3643a9