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Fabrication of engineered tubular tissue for small blood vessels via three-dimensional cellular assembly and organization ex vivo
- Source :
- Journal of biotechnology.
- Publication Year :
- 2018
-
Abstract
- Although there is a great need for suitable vascular replacements in clinical practice, much progress needs to be made toward the development of a fully functional tissue-engineered construct. We propose a fabrication method of engineered tubular tissue for small blood vessels via a layer-by-layer cellular assembly technique using mouse smooth muscle cells, the construction of a poly-(l-lactide-co-e-caprolactone) (PLCL) scaffold, and integration in a microfluidic perfusion culture system. The cylindrical PLCL scaffold is incised, expanded, and its surface is laminated with the cell layers. The construct confirms into tubular structures due to residual stress imposed by the cylindrical PLCL scaffold. The perfusion culture system allows simulation of static, perfusion (laminar flow), and perfusion with pulsatile pressure (Pulsatile flow) conditions in which mimicking the in vivo environments. The aim of this evaluation was to determine whether fabricated tubular tissue models developed their mechanical properties. The cellular response to hemodynamic stimulus imposed by the dynamic culture system is monitored through expression analysis of fibrillin-1 and fibrillin-2, elastin and smooth muscle myosin heavy chains isoforms transcription factors, which play an important role in tissue elastogenesis. Among the available materials for small blood vessel construction, these cellular hybrid vascular scaffolds hold much potential due to controllability of the mechanical properties of synthetic polymers and biocompatibility of integrated cellular components.
- Subjects :
- 0301 basic medicine
Scaffold
Materials science
Biocompatibility
Myocytes, Smooth Muscle
Pulsatile flow
Cell Culture Techniques
Bioengineering
02 engineering and technology
Applied Microbiology and Biotechnology
03 medical and health sciences
Perfusion Culture
Myosin
Animals
Aorta
Cells, Cultured
biology
Tissue Engineering
General Medicine
Equipment Design
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
Rats
Perfusion
030104 developmental biology
biology.protein
Blood Vessels
0210 nano-technology
Elastin
Ex vivo
Biotechnology
Biomedical engineering
Subjects
Details
- ISSN :
- 18734863
- Database :
- OpenAIRE
- Journal :
- Journal of biotechnology
- Accession number :
- edsair.doi.dedup.....a67dbcc2b48562f7b6f388cc7b393aa1