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Hsa_circ_0043278 functions as competitive endogenous RNA to enhance glioblastoma multiforme progression by sponging miR-638
- Source :
- Aging (Albany NY)
- Publication Year :
- 2020
- Publisher :
- Impact Journals, LLC, 2020.
-
Abstract
- Circular RNAs have a critical function in the pathogenesis of many diseases and can function as competing endogenous RNA or miRNA sponges to inhibit miRNA and therefore upregulate the expression of target genes. However, little is known about the role of has_circRNA_0043278 (circ_0043278) in glioblastoma multiforme (GBM) and its potential downstream miRNA targets. This work validated that circ_0043278 is highly expressed in GMB cell lines and tissues, while knockdown circ_0043278 inhibited GBM cell migration, proliferation, and invasion in vitro and tumorigenesis in vivo. Dual-luciferase reporter assay determined that circ_0043278 directly sponged miR-638 to upregulate the expression of HOXA9, which can activate downstream Wnt/β-catenin signaling in GBM. Moreover, miR-638 inhibition reversed circ_0043278 silencing-induced impairment of malignant tumor behavior. These results showed that circ-0043278/miRNA-638/ Homeobox A9 (HOXA9) axis had a vital function in promoting GBM progression. Our findings may provide potential new targets for the diagnosis and therapy of GBM.
- Subjects :
- Aging
circ_0043278
Biology
migration
medicine.disease_cause
glioblastoma multiforme
Downregulation and upregulation
Cell Line, Tumor
microRNA
medicine
Humans
Homeodomain Proteins
Gene knockdown
Brain Neoplasms
Competing endogenous RNA
Wnt signaling pathway
RNA
RNA, Circular
Cell Biology
invasion
Gene Expression Regulation, Neoplastic
MicroRNAs
homeobox A9
Disease Progression
Cancer research
Glioblastoma
Carcinogenesis
Research Paper
Wnt/β-catenin signaling
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.doi.dedup.....a679782332207f803c2d73cc4d303298